Identification of Human Mononuclear Cell Population by Acid alpha-Naphthyl Acetate Esterase Staining

Lee, S G; Choi, B H; Han, K Ch; Chung, T H

Abstract

This study was carried out to determine the clinical usage of acid alpha-naphthyl acetate esterase staining technic to define subpopulation of human peripheral blood lymphocytes and monocytes. 18 samples of peripheral blood smear of normal subjects were fixed in the formalin-aceton buffer fixative an stained by alpha-naphthyl acetate esterase techinc to be differentiated into 3 types of mononuclear cells; granular, diffuse, and negative patterns. As a marker for T-cell we employed E-rosette technic and for B-cell immunoenzymatic assay using alkalinephosp-hatase-antibody for human IgG complex, and for monocyte peroxidase staining method. The results from above technics were compared. Appearance of E-rosette forming cell was 61.8+/-4.92%, cells showing granular pattern by acid alpha-naphthyl-acetate esterase 71.9+/-6.35%, diffuse pattern 12.3+/-3.70%, and negative pattern 15.8+/-6.51%. Population of staining were monocytes drtermined by peroxidase method and cells showing diffuse pattern by acid alpha naphthyl-acetate esterase technic were identical. Mononuclear cells separated from 4 patients showing decreased E-rosette forming cells revealed decreased granular pattern cells. According to this study acid alpha-naphthyl acetate esterase staining technic for differentiating the sub-population of mononclear cells from peripheral bloom may play a useful role in clinical medicine.