Study on the Fluorescent Body (F-body) of the Human Spermatozoa

Shin, Dong Chull; Lee, Moo Sang

Abstract

In this study seminal fluid samples were obtained from 20 normal adult males to know the percentage of the spermatozoa containing the fluorescent body (F-body), after staining the seminal fluid with 0.5% aqueous quinacrine HCI. In this study the preparations were observed with an AO fluorescent microscope. The following results were obtained. 1) In 20 men, the mean percentage of F-body containing spermatozoa was 31.3%. 2) There was no relationship between sperm density in semen analysis and F-body rate 3) There was no relationship between sperm motility in semen analysis and F-body rate. 4) In 5 men, methyl alcohol-acetic acid (3:1) preparations, as a fixation solution, showed better F-body rate than that of ethyl alcohol preparations. 5) In 4 men, the preparations which were fixed and stained within 2 hours after obtaining seminal fluid showed better F-body rate than that fixed and stained after 6 hours. 6) In 10 men, after low speed centrifuging of the seminal fluid, the deposit preparations, showed decreased mean percentage of F-body containing spermatozoa by 4.2%.