Korean J Med.  2004 Apr;66(4):397-403.

Experimental interstitial photodynamic therapy of the Morris 7777 hepatoma in the rat

• Cheon YK
• Kim YS
• Cho YD
• Kim YS
• Lee JS
• Lee MS
• Shim CS
• Kim BS
• Jin SY

Abstract

BACKGROUND: Photodynamic therapy (PDT) of hepatic tumors has been restricted due to the preferential retention of photosensitizers in normal liver tissue. Therefore, superficial tumor illumination causes subsequential liver necrosis. Moreover, the limited light penetration during superficial illumination makes it impossible to treat deep-seated or larger solid tumors. These limitations were overcome by interstitial therapy. Aim: The aim of study is to investigate the macroscopic and microscopic effect of a single session of interstitial photodynamic therapy on liver tumor.
METHODS
The Morris 7777 hepatoma cells were injected subcutaneously into the flanks of Buffalo female rats. Tumor growth was monitored with measuring the tumor size. Animals were pretreated with hematoporphyrin derivative 48 hours prior to the intratumoral delivery of laser radiation, when the tumor had reached a volume greater than 1.0 cm3. One or two weeks after interstitial PDT, the extent of tumor necrosis was evaluated microscopically.
RESULTS
Volume of lesions averaged 10.2 mm3. Histologic stains demonstrated microvascular thrombosis and coagulative necrosis within the lesions. There appeared to be 100% cellular destruction within the lesion by cytochemical staining.
CONCLUSION
The results of this study show the ability of interstitial PDT to cause destruction of liver tumor.

Keyword

Morris 7777 hepatoma cell; Interstitial photodynamic therapy; Hematoporphyrin derivative

MeSH Terms

Animals
Buffaloes
Carcinoma, Hepatocellular*
Coloring Agents
Female
Hematoporphyrin Derivative
Humans
Lighting
Liver
Necrosis
Photochemotherapy*
Photosensitizing Agents
Rats*
Thrombosis
Coloring Agents
Hematoporphyrin Derivative
Photosensitizing Agents