Korean J Clin Microbiol.
2003 Sep;6(2):114-118.
Molecular Characterization of Vancomycin-Resistant Enterococci Isolated from Poultry in Korea
- Affiliations
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- 1Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, Korea. heidi2030@hanmail.net
- 2Department of Laboratory Medicine, Bestian Medical Center, Seoul, Korea.
- 3Division of Gastroenterology, Department of Internal Medicine, Sooncheonhyang University Cheonan Hospital, Cheonan, Korea.
- 4Department o f Laboratory Medicine, Yonsei University School of Medicine, Korea.
- 5Division of Infectious Disease, Department of Internal Medicine, Catholic University, Seoul, Korea.
Abstract
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BACKGROUND: Vancomycin-resistant enterococci (VRE) have been increasingly isolated worldwide as a nosocomial pathogen. In Korea, because avoparcin has been used as a growth promoter in animal feed, vanA-containing enterococci have been found in animals. The aim of this study is to understand the epidimiology of VRE isolated from chicken of diverse geographic areas.
METHODS
Thirty eight isolates of VanA VRE from chicken of diverse geographic areas were investigated. Multiplex PCR was used to confirm the genotype of VRE. Long PCR and restriction fragment length polymorphism (long PCR-RFLP) were performed to analyze the structure of Tn1546. If the RFLP pattern was different from the prototype, PCR amplification of internal regions of Tn1546 and subsequent sequencing analysis was performed.
RESULTS
All 38 isolates harbored vanA gene and divided into 3 types by long PCR-RFLP. Thirty five isolates (92%) were classified as type I. Two isolates and one isolate were belonging to type II and type III, respectively. Type II revealed an insertion of IS1216V in vanX-vanY intergenic region. IS element integrated type III did not match any previously reqorted seguence.
CONCLUSION
Structural analysis of vanA gene cluster from chicken revealed that the majority of isolates (type I) have indistinguishable structure to E. fecium BM4147. This results indicated horizontal spread of resistance gene among isolates from chicken. Genetic rearrangement of Tn1546 was infrequently detected in Korea.
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