Korean J Phys Anthropol.
2006 Sep;19(3):179-189.
Effects of Repetitive Ischemic Preconditioning on the Expression of Cu,ZnSOD and MnSOD in Rectus Femoris Muscles of Rats
- Affiliations
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- 1Department of Anatomy and Cell biology, College of Medicine, Hanyang University, Korea. paikdj@hanyang.ac.kr
Abstract
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The present study was performed to investigate the dynamics of Cu,ZnSOD and MnSOD expression following
courses of reperfusion after repetitive ischemic preconditioning on the left rectus femoris muscle of Spraque-Dawley
rats.
Nine or thirty five weeks-old rats were subjected to three, six and ten cycles of ischemic preconditioning that was 5
min ischemia and 5 min reperfusion at the left common iliac artery. Left rectus femoris muscle was isolated 0, 3, 6, 24
and 72 hours of reperfusion after ischemic preconditioning and assayed by immunohistochemical staining with anti-Cu,ZnSOD and anti-MnSOD antibodies. The results were as follows;
The immunoreactivities of Cu,ZnSOD and MnSOD were increased in the repectitive three and six cycles of ischemic
preconditioning. However, after the repetitive ten cycles of ischemic preconditioning, the Cu,ZnSOD immunoreactivities
were decreased in the nine weeks-old rats while MnSOD immunoreactivities were decreased in thirty five
weeks-old rats.
These findings suggest that severe damayes result from decrease of Cu,ZnSOD in nine weeks-old rats and decrease
of MnSOD in thirty five weeks-old rats after ten cycles of ischemic preconditioning.