J Bacteriol Virol.  2005 Sep;35(3):191-196.

Phage Typing of Staphylococcus intermedius Isolated from Canine Clinical Specimens

• Park CK
• Kim SK
• Lee YJ
• Yeo SG
• Kim KS

Abstract

Bacteriophages were induced from staphylococcus intermedius isolates from dogs and used for a phage typing. Mitomycin C induction was performed on 60 strains of S. intermedius and all phages were reacted with the strains. Twenty-eight strains (46.7%) were found to be lysogenic. Based on host ranges, eight phages were selected. By using the eight phages, 129 strains isolated from canine clinical specimens were subjected to the phage typing at the routine test dilution (RTD) and 100xRTD. Typability of the phage set was 78.3%, yielding 40 phage patterns. The phage set was considered useful for differentiating S. intermedius strains isolated from dogs. None of 50 strains of S. aureus, 2 (3.6%) of 56 S. simulance strains, 13 (24.5%) of 53 S. chromogenes strains and 46 (28.1%) of 164 S. hyicus strains were typable by the phages at 100xRTD.

Keyword

Staphylococcus intermedius; Dogs; Phage typing

MeSH Terms

Animals
Bacteriophage Typing*
Bacteriophages*
Dogs
Host Specificity
Mitomycin
Staphylococcus intermedius*
Staphylococcus*
Mitomycin