Korean J Fertil Steril.  2002 Sep;29(3):187-194.

The Analysis of Interrelationship between Homocysteine and Methylenetetrahydrofolate Reductase Mutation in Patients with Recurrent Spontaneous Abortion

• Nam YS
• Cha KY
• Kim NK
• Kang MS
• Kim SH
• Oh D

Abstract

OBJECTIVE
To analyze the interrelationship between homocysteine and methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. MATERIAL AND METHOD: Homocysteine and MTHFR mutation were tested by fluorescent polarizing immunoassay and PCR-RFLP method, respectively.
RESULTS
In patients with homocysteine level less than 5 mmol/L, there was no case of normal group but there were four cases of heterozygosity and one case of homozygosity. In patients with homocysteine level 5~10 mmol/L, the number of normal, heterozygosity and homozygosity group were eleven, eighteen and eight, respectively. In patients with homocysteine level 10~15 mmol/L, the number of normal, heterozygosity and homozygosity group were four, one and one, respectively. In patients with homocysteine level more than 15 mmol/L, there was no case of normal and heterozygosity group but there were two cases of homozygosity.
CONCLUSIONS
Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. And there was a significant relationship between homocysteine and MTHFR mutation.

Keyword

Recurrent spontaneous abortion; Homocysteine; MTHFR mutation

MeSH Terms

Abortion, Spontaneous*
Female
Homocysteine*
Humans
Hyperhomocysteinemia
Immunoassay
Methylenetetrahydrofolate Reductase (NADPH2)*
Pregnancy
Homocysteine
Methylenetetrahydrofolate Reductase (NADPH2)