J Korean Soc Virol.
1999 Sep;29(3):175-182.
Expression and Purification of Herpes Simplex Virus Type 1 Protease
- Affiliations
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- 1Pharmaceutical Screening Center, Korea Research Institute of Chemical Technology, Taejon 305-600, Korea.
- 2Department of Biology, Chungnam National University, Taejon, Korea.
- 3Department of Biology, Yonsei University, Seoul, Korea.
Abstract
- An attractive target for anti-herpes chemotherapy is the herpes simplex virus 1 (HSV-1) protease encoded by the UL26 gene. HSV-1 protease is essential for DNA packaging and virus maturation. To perform high throughput for potent inhibitors, the efficient production of larger amounts of highly purified enzyme and protease activity assay method must be established. In this report, expression in E. coli and purification of the protease gene of HSV-1 strain F was investigated. The protease gene was cloned pET28, and the nucleotide sequence of protease catalytic domain of HSV-1 compared strain F with other strains (KOS and CL101). In these results the F strain was different in base sequence. However, the amino acid sequence was identifical. The HSV-1 protease was purified with His-tagged affinity column. The analysis of HSV-1 protease activity Was performed by high performance liquid chromatography.
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