Genomics Inform.  2006 Sep;4(3):97-102.

Identification of Gene Expression Signatures in Korean Acute Leukemia Patients

• Lee KH
• Park SW
• Kim I
• Yoon SS
• Park S
• Kim BK

Abstract

BACKGROUND
In acute leukemia patients, several successful methods of expression profiling have been used for various purposes, i.e., to identify new disease class, to select a therapeutic target, or to predict chemo-sensitivity and clinical outcome. In the present study, we tested the peripheral blood of 47 acute leukemia patients in an attempt to identify differentially expressed genes in AML and ALL using a Korean-made 10K oligo-nucleotide microarray. METHODS: Total RNA was prepared from peripheral blood and amplified for microarray experimentation. SAM (significant analysis of microarray) and PAM (prediction analysis of microarray) were used to select significant genes. The selected genes were tested for in a test group, independently of the training group. RESULTS: We identified 345 differentially expressed genes that differentiated AML and ALL patients (FWER < 0.05). Genes were selected using the training group (n=35) and tested for in the test group (n=12). Both training group and test group discriminated AML and ALL patients accurately. Genes that showed relatively high expression in AML patients were deoxynucleotidyl transferase, pre-B lymphocyte gene 3, B-cell linker, CD9 antigen, lymphoid enhancer-binding factor 1, CD79B antigen, and early B-cell factor. Genes highly expressed in ALL patients were annexin A 1, amyloid beta (A4) precursor protein, amyloid beta (A4) precursor-like protein 2, cathepsin C, lysozyme (renal amyloidosis), myeloperoxidase, and hematopoietic prostaglandin D2 synthase. CONCLUSION: This study provided genome wide molecular signatures of Korean acute leukemia patients, which clearly identify AML and ALL. Given with other reported signatures, these molecular signatures provide a means of achieving a molecular diagnosis in Korean acute leukemia patents.

Keyword

acute myeloid leukemia; acute lymphoblastic leukemia; microarray; expression profiling

MeSH Terms

Amyloid
Antigens, CD79
Antigens, CD9
B-Lymphocytes
Cathepsin C
Diagnosis
DNA Nucleotidylexotransferase
Gene Expression*
Genome
Humans
Leukemia*
Leukemia, Myeloid, Acute
Lymphoid Enhancer-Binding Factor 1
Muramidase
Peroxidase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Precursor Cells, B-Lymphoid
Prostaglandin D2
RNA
Transcriptome*
Amyloid
Antigens, CD79
Antigens, CD9
Cathepsin C
DNA Nucleotidylexotransferase
Lymphoid Enhancer-Binding Factor 1
Muramidase
Peroxidase
Prostaglandin D2
RNA