Korean J Fertil Steril.  2006 Mar;33(1):53-60.

Comparison between Human Follicular Fluid and SpermGrad for Sperm Preparation in Asthenozoospermia

• Chung YK
• Lee JR
• Moon JH
• Kim HJ
• Han SH
• Jee BC
• Ku SY
• Suh CS
• Kim SH
• Choi YM
• Kim JG
• Moon SY

Abstract

OBJECTIVE
We tested the usefulness of swim-down technique using human follicular fluid (hFF) in sperm preparation.
METHODS
Semen samples were obtained from twelve male partners showing asthenozoospermia (sperm motility < 50%) at the time of routine andrologic evaluation in Seoul National University Bundang Hospital. After dividing into two aliquots, each samples were processed either by swim-down using 100% hFF or density gradient using SpermGrad. Sperm quality was assessed by computer-assisted semen analyzer (CASA).
RESULTS
Motility, Rapid motility, VCL (curvilinear velocity), ALH (amplitude of lateral head displacement), and hyperactivated sperms were significantly increased, and LIN (mean linearity) was decreased significantly after sperm preparation in both groups. Motility was significantly higher after swim-down using 100% hFF when compared with density gradient using SpermGrad (81.2+/-4.7 vs. 67.6+/-2.3, p=0.02). The other parameters assessed by CASA were not different between the two methods.
CONCLUSION
Swim-down method with 100% hFF may be a useful method in preparation of sperm from asthenozoospermia.

Keyword

Asthenozoospermia; Density gradient; Human follicular fluid; SpermGrad; Swim-down

MeSH Terms

Asthenozoospermia*
Female
Follicular Fluid*
Head
Humans*
Male
Semen
Seoul
Spermatozoa*