Yonsei Med J.  2013 Mar;54(2):476-479. 10.3349/ymj.2013.54.2.476.

Evaluation of a Rapid Detection Influenza Virus A Antigens Kit Using Paired Serum Antibody Test

• Chen H
• Wang H
• Li X
• You L
• Wei J
• Shi W

• KMID: 1503913
• DOI: http://doi.org/10.3349/ymj.2013.54.2.476

Abstract

PURPOSE
To evaluate the feasibility for gold immunochromatographic assay (GICA) in rapid detection of influenza virus A infection.
MATERIALS AND METHODS
Seventy-three patients were enrolled. All patients contributed nasopharyngeal secretions and paired serum samples. Nasopharyngeal secretions was used for colloidal gold immunochromatographic rapid assay for influenza A virus immediately after the collection of specimen. Paired serum samples were used for the hemagglutination inhibition assay at the Centers for Disease Control and Prevention influenza network laboratory in Beijing.
RESULTS
Compare GICA test to hemagglutination inhibition (HI) assay, the Kappa value was 0.402 and the p value in the paired chi2 test was higher than 0.05. Therefore, the difference was not statistically significant. The sensitivity of GICA was 50.0% and the specificity was 90.2%, and the negative predictive value was 90.2%.
CONCLUSION
The sensitivity for Influenza A antigen detection by using GICA is relatively low, the specificity is relatively satisfactory.

Keyword

Influenza A virus; colloidal gold immunochromatographic assay; antibody; rapid test

MeSH Terms

Adult
Aged
Aged, 80 and over
Antigens, Viral/*blood
Female
*Gold Colloid
Humans
Immunochromatography/*methods
Influenza A virus/*immunology
Influenza, Human/*diagnosis/immunology
Male
Middle Aged
Reagent Kits, Diagnostic
Sensitivity and Specificity
Antigens, Viral
Gold Colloid
Reagent Kits, Diagnostic

Figure

• 

  Fig. 1 Direct-viewing chart for the detection results by using GICA. GICA, gold immunochromatographic assay.

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