Creatinine Interference dependent on Glucose and Creatinine Concentrations

Park, Dong Jin; Park, Ji Young; Cho, Hyoun Chan

Abstract

BACKGROUND
In peritoneal dialysis patients, measurement of creatinine is an important marker of kidney function and gives an information for assessment of dialytic adequacy. High glucose concentration in peritoneal dialysis fluid is known to interfere with creatinine measurement. Creatininine interference with kinetic Jaffe method for glucose and creatinine concentration must be considerated for giving accurate informations about the assessment of dialytic adequacy.
METHODS
10% dextrose fluid (Daihan Pharm Co., Korea) was diluted to prepare specimens with seven different glucose concentrations. Creatinine solutions with seven different concentrations were made with creatinine powder (Sigma-Aldrich Co., USA) and distilled water. The prepared specimens were mixed with equal volume to make total 49 specimens of different glucose and creatinine concentrations. The glucose concentrations of specimens were ranging from 200 mg/dL to 5,000 mg/dL and the creatinine concentrations of specimens were ranging from 0 mg/dL to 10 mg/dL. The specimens were assayed for creatinine with two automated chemistry analysers, Hitachi 7600-110 (Hitachi, Japan) and Unicel DXC 800 (Beckman Coulter Inc., USA). Creatinine HR reagent (Wako Pure Chemical Industries, Japan) and CREA reagent (Roche Diagnostics, Germany) were used in Hitachi 7600-110 analyser, and CREm reagent (Beckman Coulter Inc., Ireland) was used in Unicel DXC 800.
RESULTS
Interference of creatinine measurement varied with both glucose and creatinine concentrations to different extent in different analytical systems and reagents. It was observed that creatinine interference increased with increasing glucose concentration in all the systems and reagents. At constant glucose concentration, creatinine interference showd a downward tendency with increasing creatinine concentration among the three reagents.
CONCLUSIONS
High glucose concentration and creatinine concentrations provoked the interference of creatinine measurement and the aspect of creatinine interference varied according to the analytic systems and reagents. Each center performing creatinine assay should allow for the creatinine interference and give an accurate results to clinicians.