Clin Exp Otorhinolaryngol.  2009 Mar;2(1):13-19. 10.3342/ceo.2009.2.1.13.

Galectin-8 Expression in Laryngeal Squamous Cell Carcinoma

• Dong GW
• Kim J
• Park JH
• Choi JY
• Cho SI
• Lim SC

• KMID: 1466555
• DOI: http://doi.org/10.3342/ceo.2009.2.1.13

Abstract

OBJECTIVES
Despite the ongoing development of treatment protocols for laryngeal squamous cell carcinoma (LSCC), the patients suffering with this malady have shown only a modestly improved outcome. This poor outcome has been attributed to the lack of therapy that's individualized to the tumor's biological properties. Various studies have showed that galectin-8 is widely expressed in tumor tissues as well as in normal tissues, and the level of the galectin-8 expression may correlate with the malignancy of human squamous cell carcinoma. The purpose of this study is to evaluate the expression of galectin-8 and to investigate its correlations with the primary stage, the nodal involvement, the clinical stage and the histologic grade of squamous cell carcinoma of the larynx.
METHODS
The paraffin-embedded tissue specimens from 77 patients who were diagnosed as LSCC between 1993 and 2007 were immunohistochemically stained for galectin-8.
RESULTS
Immunohistochemical analysis showed that a strong positive expression of galectin-8 was correlated with the T-stages, the nodal stages and the clinical stages. However, the histopathologic grades were not correlated with the galectin-8 expression in LSCC.
CONCLUSION
The expression of galectin-8 protein can be used as a prognostic factor for patients with LSCC.

Keyword

Galectin-8; Immunohistochemistry; Larynx; Squamous cell carcinoma

MeSH Terms

Carcinoma, Squamous Cell
Clinical Protocols
Humans
Immunohistochemistry
Larynx
Stress, Psychological

Figure

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  Fig. 1 Immunohistochemical staining of galectin-8 in laryngeal squamous cell carcinoma shows intense immunoreactivity (arrows), and particularly in the stromal invasive region (LSAB method, counterstained by Mayer's hematoxylin, ×200).

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  Fig. 2 Immunohistochemical staining of galectin-8 in laryngeal squamous cell carcinoma. A strong galectin-8 expression (arrows) is identified in the squamous cell carcinoma (lower portion) as compared with the normal and dysplastic squamous epithelium (upper portion) (LSAB method, counterstained by Mayer's hematoxylin, ×200).

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  Fig. 3 Galectin-8 encodes for a galectin with two homologous carbohydrate-binding regions. A schematic structure of galectin-8 is presented. Each box represents a putative carbohydrate-binding domain, linked by a 32 amino acid-long peptide. Also shown are selected invariant amino acids that are preserved in most of the galectins that have currently been analyzed.

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