J Bacteriol Virol.  2011 Sep;41(3):157-164. 10.4167/jbv.2011.41.3.157.

Correlation Between the Prevalence of Superantigenic Toxin Genes and Coagulase Serotypes of Staphylococcus aureus Isolates

Affiliations
  • 1Department of Clinical Laboratory Science, Catholic University of Pusan, Busan, Korea. smhwang@cup.ac.kr

Abstract

A heterogenic group of staphylococcal exotoxins, including staphylococcal superantigenic toxins, enterotoxin (SE), toxic shock syndrome toxin-1 (TSST-1), and coagulase are the most important virulence factors of Staphylococcus aureus. We analyzed the prevalence of genes encoding five enterotoxins and TSST-1 in S. aureus isolated from clinical ear discharges. The genes were identified by multiplex PCR and we compared the results to references of coagulase serotypes. In 102 isolates of S. aureus, 44 of them were methicillin-resistant S. aureus (MRSA) and the others were methicillin-susceptible S. aureus (MSSA). Among both types of S. aureus, 33 strains were positive for sea, 2 for seb, 23 for sec, 26 for see, and 26 for tst. Overall, 59 (57.8%) isolates were positive for one or more superantigenic toxin genes. From these, 71.2% (42/59) strains harbored more than one toxin gene in different combinations. The major combinations of genes were sea and see, and sec and tst. The degree of possession of superantigenic toxic genes was similar in both MRSA and MSSA isolates (56.8% vs 58.6%, respectively), yet significant differences in toxin gene profiles and coagulase serotypes between two isolates were detected. All of 13 positive strains for sec and tst were MRSA and belonged to coagulase serotype II. On the other hand, 80.0% of 20 positive strains for sea and see were MSSA with coagulase serotype IV and VII, whereas 20.0% of them were MRSA with coagulase serotype IV. This data indicates that the profile of superantigenic toxin genes correlates to coagulase serotype and methicillin resistance in S. aureus isolates.

Keyword

Staphylococcus aureus; Staphylococcal enterotoxins; Toxic shock syndrome toxin-1; Coagulase serotype

MeSH Terms

Bacterial Toxins
Coagulase
Ear
Enterotoxins
Exotoxins
Hand
Methicillin Resistance
Methicillin-Resistant Staphylococcus aureus
Multiplex Polymerase Chain Reaction
Prevalence
Shock, Septic
Staphylococcus
Staphylococcus aureus
Superantigens
Virulence Factors
Bacterial Toxins
Coagulase
Enterotoxins
Exotoxins
Superantigens
Virulence Factors
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