Nucl Med Mol Imaging.  2011 Dec;45(4):248-254.

Radiosynthesis of 99mTc(CO)3-Clinafloxacin Dithiocarbamate and Its Biological Evaluation as a Potential Staphylococcus aureus Infection Radiotracer

• Shah SQ
• Khan MR
• Ali SM

Abstract

BACKGROUND
Clinafloxacin dithiocarbamate (CNND) was radiolabeled with technetium-99m (99mTc) using [99mTc(CO)3(H2O)3]+ and assessed for its radiochemical stability in saline and serum, its in vitro binding with methicillin-resistant Staphylococcus aureus (MRSA) and biodistribution in female nude mice (FNM) artificially infected with live and heat-killed MRSA.
METHODS
In normal saline (NS) the 99mTc(CO)3-clinafloxacin dithiocarbamate (99mTc(CO)3-CNND) showed radiochemical stability with a maximum value of 99.10+/-0.20% and remained stable up to 4 h (92.65+/-0.18%).
RESULTS
In human serum at 37degrees C within 16 h of incubation, 14.85% side products as a result of de-tagging developed. Incubation with MRSA gave saturated binding with a maximum value of 72.75+/-1.20%. Almost six-fold higher uptake was seen in the infected muscle of the FNM as compared to the inflamed and normal muscle. The 99mTc(CO)3-CNND complex showed a normal route of excretion from the body of the FNM model.
CONCLUSION
The higher stability in NS, HS, saturated in vitro binding with a live strain ofMRSA and six-fold higher uptake in the target organ showed the 99mTc(CO)3-CNND complex to be a potential MRSA infection radiotracer.

Keyword

99mTc(CO)3-Clinafloxacin dithiocarbamate complex; MRSA; Infection radiotracer

MeSH Terms

Animals
Female
Fluoroquinolones
Humans
Methicillin-Resistant Staphylococcus aureus
Mice
Mice, Nude
Muscles
Sprains and Strains
Staphylococcus
Staphylococcus aureus
Fluoroquinolones