Lab Med Online.  2011 Apr;1(2):94-99. 10.3343/lmo.2011.1.2.5.

Evaluation of an Automated Coagulation Analyzer Coapresta 2000

Affiliations
  • 1Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. labdx@yuhs.ac

Abstract

BACKGROUND
The Coapresta 2000 (Sekisui Medical CO., LTD, Japan) is a fully automated random-access multiparameter hemostasis coagulation analyzer, which is equipped with a photo-optical clot detection unit and a cap-piercing system. It is able to perform clotting time assays as well as colorimetric assays (synthetic substrate method and latex turbidimetric method). In this study, we evaluated the analytical performance of the Coapresta 2000 for coagulation test items and compared with that of the ACL-TOP (Instrumentation Laboratory, Lexingtion, MA, USA) analyzer, which is currently used for routine coagulation test items in our hospital.
METHODS
The Coapresta 2000 was evaluated with respect to its technical characteristics in the determination of 8 routine coagulation test items: prothrombin time, activated partial thromboplastin time, fibrinogen, fibrin-degradation product (FDP) antithrombin III, D-dimer, factors VIII and IX. Analyse-it (Analyse-it Software Ltd, UK) and SigmaStat (Systat Software, Inc., USA) were used for statistical analysis between items on the Coapresta 2000 and the ACL-TOP analyzer.
RESULTS
The intra-assay and inter-assay coefficients of variation (CV) were below 5% for both groups of samples having values within the reference interval and outside the reference interval. Significant interference was observed with hemolytic and icteric samples. Carryover was not detected. The results obtained by Coapresta 2000 were well correlated with those obtained by the ACL-TOP analyzer (r2 in the range from 0.781 to 0.969).
CONCLUSIONS
We concluded that Coapresta 2000 analyzer was well correlated with ACL-TOP analyzer for the routine coagulation test items tested.

Keyword

Coapresta 2000; Evaluation; Correlation; ACL-TOP

MeSH Terms

Antithrombin III
Fibrin Fibrinogen Degradation Products
Fibrinogen
Hemostasis
Latex
Partial Thromboplastin Time
Prothrombin Time
Antithrombin III
Fibrin Fibrinogen Degradation Products
Fibrinogen
Latex

Figure

  • Fig. 1 Correlation of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.

  • Fig. 2 Bias of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.


Reference

1. Walenga JM, Fareed J. Automation and quality control in the coagulation laboratory. Clin Lab Med. 1994. 14:709–728.
Article
2. Amiral J, Adalbert B, Adam M. Application of enzyme immunoassays to coagulation testing. Clin Chem. 1984. 30:1512–1516.
Article
3. Fischer F, Appert-Flory A, Jambou D, Toulon P. Evaluation of the automated coagulation analyzer Sysmex CA-7000. Thromb Res. 2006. 117:721–729.
Article
4. Appert-Flory A, Fischer F, Jambou D, Toulon P. Evaluation and performance characteristics of the automated coagulation analyzer ACL TOP. Thromb Res. 2007. 120:733–743.
Article
5. Kim YA, Sun YK. Measurement of PT, aPTT, and fibrinogen by automatic coagulation analyzer, ACL9000. J Lab Med Qual Assur. 2002. 24:201–207.
6. Kim HS, Nam KH, Lee DH. Measurement of PT, APTT, and fibrinogen by automatic coagulation analyzer, Sysmex CA-540. J Clin Pathol Qual Control. 2001. 23:247–252.
7. Lee YW, Chang CW, Lim MS, Lim BJ, Lee YK. Laboratory evaluation of automated coagulation analyzers sysmex CA-1500TM and CA-7000TM. J Clin Pathol Qual Control. 2001. 23:253–258.
8. Clinical and Laboratory Standards Institute. CLSI Document H47-A2. One-stage prothrombin time (PT) test and activated partial thromboplastin time (APTT) test; approved guideline. 2008. Wayne, PA: Clinical and Laboratory Standards Institute.
9. National Committee for Clinical Laboratory Standards. NCCLS Document H30-A2. Procedure for the determination of fibrinogen in plasma; approved guideline-second edition. 2001. Wayne, PA: National Committee for Clinical Laboratory Standards.
10. Milos M, Herak D, Kuric L, Horvat I, Zadro R. Evaluation and performance characteristics of the coagulation system: ACL TOP analyzer-HemosIL reagents. Int J Lab Hematol. 2009. 31:26–35.
Article
11. Eschwège V, Catillon C, Robert A. Evaluation of the automated coagulation analyser ACL TOP (instrumentation laboratory). Ann Biol Clin (Paris). 2006. 64:259–264.
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