Lab Med Online.  2011 Apr;1(2):94-99. 10.3343/lmo.2011.1.2.5.

Evaluation of an Automated Coagulation Analyzer Coapresta 2000

• Choi J
• Song S
• Park Y
• Choi JR
• Song J

• KMID: 1446275
• DOI: http://doi.org/10.3343/lmo.2011.1.2.5

Abstract

BACKGROUND
The Coapresta 2000 (Sekisui Medical CO., LTD, Japan) is a fully automated random-access multiparameter hemostasis coagulation analyzer, which is equipped with a photo-optical clot detection unit and a cap-piercing system. It is able to perform clotting time assays as well as colorimetric assays (synthetic substrate method and latex turbidimetric method). In this study, we evaluated the analytical performance of the Coapresta 2000 for coagulation test items and compared with that of the ACL-TOP (Instrumentation Laboratory, Lexingtion, MA, USA) analyzer, which is currently used for routine coagulation test items in our hospital.
METHODS
The Coapresta 2000 was evaluated with respect to its technical characteristics in the determination of 8 routine coagulation test items: prothrombin time, activated partial thromboplastin time, fibrinogen, fibrin-degradation product (FDP) antithrombin III, D-dimer, factors VIII and IX. Analyse-it (Analyse-it Software Ltd, UK) and SigmaStat (Systat Software, Inc., USA) were used for statistical analysis between items on the Coapresta 2000 and the ACL-TOP analyzer.
RESULTS
The intra-assay and inter-assay coefficients of variation (CV) were below 5% for both groups of samples having values within the reference interval and outside the reference interval. Significant interference was observed with hemolytic and icteric samples. Carryover was not detected. The results obtained by Coapresta 2000 were well correlated with those obtained by the ACL-TOP analyzer (r2 in the range from 0.781 to 0.969).
CONCLUSIONS
We concluded that Coapresta 2000 analyzer was well correlated with ACL-TOP analyzer for the routine coagulation test items tested.

Keyword

Coapresta 2000; Evaluation; Correlation; ACL-TOP

MeSH Terms

Antithrombin III
Fibrin Fibrinogen Degradation Products
Fibrinogen
Hemostasis
Latex
Partial Thromboplastin Time
Prothrombin Time
Antithrombin III
Fibrin Fibrinogen Degradation Products
Fibrinogen
Latex

Figure

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  Fig. 1 Correlation of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.

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  Fig. 2 Bias of the test results between ACL-TOP and CP 2000 analyzers for 8 coagulation test items.

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