Korean J Vet Res.  2011 Jun;51(2):129-137.

Identification of two cytopathogenic agents, Mycoplasma hyorhinis and mammalian orthoreovirus 3 based on modified particle associated nucleic acids PCR

• Kim HK
• Moon HJ
• Park SJ
• Rho SM
• Han JY
• Nguyen VG
• Park BK

Abstract

Swine diseases could be caused by unrecognized or minor pathogens. In this study, two unknown cytopathogenic agents were isolated from swine, through cell culture. In order to identify these two cytopathogenic agent (designated CP129 and #2045-7), a particle associated nucleic acids PCR (PAN-PCR) from previous paper was used with simple modification. The cloning procedure was more specified in this study by adding cell control system. According to the modified PAN-PCR, two and four agents-specific DNA sequences were obtained from CP129 and #2045-7, respectively, and they were identified as Mycoplasma (M.) hyorhinis and Mammalian orthoreovirus by nucleotide BLAST. Since M. hyorhinis (CP129) was filterable and non-visible by microscope, this unusual virus-like nature of M. hyorhinis (CP129) was discussed. Especially, the reovirus (#2045-7) was a serotype 3 and a triple reassortant among three serotypes of reoviruses. It was grouped with recently reported reoviruses from disease cases (swine, human and feline), based on the genetic analysis of L1 and S1 partial sequences. In conclusion, two unknown cytopathogenic agents were successfully identified using modified PAN-PCR with cell control system and they were characterized in this study.

Keyword

mammalian orthoreovirus; Mycoplasma hyorhinis; PAN-PCR; pig; unknown

MeSH Terms

Base Sequence
Cell Culture Techniques
Clone Cells
Cloning, Organism
Humans
Mammalian orthoreovirus 3
Mycoplasma
Mycoplasma hyorhinis
Nucleic Acids
Orthoreovirus, Mammalian
Polymerase Chain Reaction
Swine
Swine Diseases
Nucleic Acids