Korean J Pathol.  2011 Jun;45(3):237-246. 10.4132/KoreanJPathol.2011.45.3.237.

Podocyte Expression of Osteopontin and FSP-1/S100A4 in Human Crescentic Glomerulonephritis

• Yoon GS
• Kim TS

• KMID: 1443728
• DOI: http://doi.org/10.4132/KoreanJPathol.2011.45.3.237

Abstract

BACKGROUND
Osteopontin (OPN) is a cytokine associated with a cell-matrix via integrins. Fibroblast specific protein-1 (FSP-1), known as S100A4, has been implicated in cell migration by non-muscle myosin. We investigated whether the role of OPN and FSP-1/S100A4 expression in their contribution to the podocyte phenotype change to form podocyte bridge and cellular crescent.
METHODS
Glomerular expression of OPN and FSP-1/S100A4 in renal biopsies of 16 patients with crescentic glomerulonephritis (CrGN) and 13 normal renal biopsies were studied by immunohistochemistry.
RESULTS
The expression of OPN and FSP-1/S100A4 was increased in the podocytes of glomeruli, with and without crescents, in patients with CrGN. Neither OPN nor FSP-1/S100A4 was expressed in glomeruli from the normal controls (p<0.01). A significant positive correlation was found between the expression of OPN in glomerular tufts and cellular crescents, and the expression of OPN and FSP-1/S100A4 in glomerular tufts (p<0.05).
CONCLUSIONS
The results suggest that OPN plays a role in early podocyte attachment to Bowman's capsule, and FSP-1/S100A4 potentiate podocyte contribution to cellular crescent formation by inducing cellular migration and growth.

Keyword

Osteopontin; FSP1 protein; S100A4 protein; Podocytes; Crescentic glomerulonephritis

MeSH Terms

Biopsy
Bowman Capsule
Cell Movement
Fibroblasts
Glomerulonephritis
Humans
Integrins
Myosins
Osteopontin
Phenotype
Podocytes
Integrins
Myosins
Osteopontin