J Korean Assoc Maxillofac Plast Reconstr Surg.  2012 Jul;34(4):239-245.

Anti-tumor Effects of Vascular Endothelial Growth Factor Receptor-3 Inhibitor on Oral Cancer Cells

• Kim CW
• Kim SG
• Park YW

Abstract

PURPOSE
Vascular endothelial growth factor (VEGF) plays a key role in tumor angiogenesis and lymphangiogenesis including induction of endothelial cell proliferation, migration and capillary tube formation. E7080 (S1164, Selleck chemical, Houston, TX, USA) is a muti-targeted kinase inhibitor, which targets VEGF receptor-2, 3 (VEGFR-2, 3) and inhibits survival and proliferation of tumor cell. The purpose of this study was to determine the anti-tumor effect of E7080 on oral squamous cell carcinoma.
METHODS
An oral squamous cell carcinoma cell line, SCC-9 was used in this study. E7080 was applied to SCC-9 cells by 3 different concentrations (1, 5, 10 microg/mL). Control means no application of E7080. The cellular growth was evaluated by real-time cell electronic sensing and MTT assay. The signal transduction was evaluated by Western blotting.
RESULTS
In experimental group, SCC-9 cell proliferation was decreased and the VEGFR-3 downstream pathways were inhibited compared with control. Furthermore, increasing the concentration of E7080, the ability of E7080 to disturbance of SCC-9 cell proliferation was increased.
CONCLUSION
Proliferation of SCC-9 cells was inhibited by E7080, which was through by inhibition of VEGFR-3 downstream pathway. In vivo study with E7080 will be required to provide therapeutic benefits in oral squamous cell carcinoma.

Keyword

Squamous cell carcinoma; VEGFR-3; E7080

MeSH Terms

Capillaries
Carcinoma, Squamous Cell
Cell Line
Cell Proliferation
Electronics
Electrons
Endothelial Cells
Lymphangiogenesis
Phosphotransferases
Signal Transduction
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-3
Phosphotransferases
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-3