Korean J Clin Microbiol.  2012 Sep;15(3):104-109. 10.5145/KJCM.2012.15.3.104.

Detection of Trichomonas vaginalis, Gardnerella vaginalis, and Candida Species in Affirm VPIII, Papanicolaou Smear Test and Gram Stain

Affiliations
  • 1Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea.
  • 2Department of Urology, Chung-Ang University College of Medicine, Seoul, Korea.
  • 3Department of Laboratory Medicine, Chung-Ang University College of Medicine, Seoul, Korea. cpworld@cau.ac.kr
  • 4Department of Pathology, Chung-Ang University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
Infectious vaginitis is caused primarily by three different groups of microbial pathogens (Trichomonas vaginalis, Candida spp., and Gardnerella vaginalis). The objective of this study was to compare the Affirm VPIII assay using a DNA hybridization technique with the Papanicolaou (Pap) smear test and the Gram stain in the detection and identification of these three organisms.
METHODS
A total of 300 vaginal samples were collected from women that were either symptomatic for vaginitis or asymptomatic women that were being seen for routine obstetric or gynecological care. The presence of T. vaginalis, Candida spp., and G. vaginalis was evaluated by using the Affirm VIII assay (Becton Dickinson, USA), Pap smear test, and Gram stain method, respectively.
RESULTS
With the Affirm VPIII assay, 1 (0.3%) patient tested positive for T. vaginalis, 99 (33.0%) patients were positive for G. vaginalis, and 18 (6.0%) were positive for Candida spp. The detection rates of Trichomonas infection, bacterial vaginosis and candidiasis by the Pap smear test and Gram stain method were 0.7% versus 0%, 16.3% versus 35.7%, and 1.7% versus 9.7%, respectively. The differences between the detection rates of the above three organisms between the Pap smear test and the Gram stain method were statistically significant (p<0.05).
CONCLUSION
The Affirm VPIII assay was more sensitive than the Pap smear test and more specific than the Gram stain method for the detection and identification of these three organisms. In addition, the results of the Affirm VPIII assay are quick to obtain and are simple and easy to interpret.

Keyword

Affirm VPIII; Candida; Gardnerella vaginalis; Gram stain; Papanicolaou stain; Trichomonas vaginalis

MeSH Terms

Candida
Candidiasis
Chimera
DNA
Female
Gardnerella
Gardnerella vaginalis
Humans
Trichomonas
Trichomonas Infections
Trichomonas vaginalis
Vaginal Smears
Vaginitis
Vaginosis, Bacterial
DNA
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