Immune Netw.  2013 Apr;13(2):43-54. 10.4110/in.2013.13.2.43.

Multiparameter Flow Cytometry: Advances in High Resolution Analysis

• O'Donnell
• Ernst DN
• Hingorani

• KMID: 1431925
• DOI: http://doi.org/10.4110/in.2013.13.2.43

Abstract

Over the past 40 years, flow cytometry has emerged as a leading, application-rich technology that supports high-resolution characterization of individual cells which function in complex cellular networks such as the immune system. This brief overview highlights advances in multiparameter flow cytometric technologies and reagent applications for characterization and functional analysis of cells modulating the immune network. These advances significantly support high-throughput and high-content analyses and enable an integrated understanding of the cellular and molecular interactions that underlie complex biological systems.

Keyword

Antibodies; Multiplex immunoassays; Multi-color flow cytometry; Fluorescent dyes; Immunophenotyping; Single cell analysis/methods

MeSH Terms

Antibodies
Flow Cytometry
Fluorescent Dyes
Immune System
Immunophenotyping
Antibodies
Fluorescent Dyes