J Lab Med Qual Assur.
2012 Dec;34(2):99-105.
The Frequency of Unexpected Antibodies and Clinical Characteristics of Transfusion Candidates in a General Hospital During the Past 12 Years
- Affiliations
-
- 1Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. yangsoon@yuhs.ac
- 2Department of Laboratory Medicine, National Health Insurance Corporation Ilsan Hospital, Goyang, Korea.
Abstract
- BACKGROUND
Unexpected antibodies are an important cause of hemolytic transfusion reaction. Thus, unexpected antibody screening and identification tests should be performed before every transfusion. We evaluated the frequency and distribution of unexpected antibodies and the clinical characteristics of patients in a Korean secondary general hospital with 745 beds in the past 12 years.
METHODS
Between March 2000 and October 2011, unexpected antibody screening and an identification test using the Bio-Rad ID-System (Bio-Rad, USA) were performed in 72,600 patients.
RESULTS
Of the 72,600 patients, 467 (0.64%) showed positive results for antibody screening tests. Among them, alloantibodies were identified in 324 (69.4%) patients and the types of alloantibodies were not identified in 64 (13.7%) patients. Autoantibodies were detected in 71 (15.2%) patients and panagglutination reactions were detected in 8 (1.7%). Of the 467 cases, 164 (35.1%) had a history of transfusion in our hospital. Among the 324 patients in whom alloantibodies were identified, anti-E (37.3%), anti-Lea (16.7%), anti-E and anti-c (14.8%), anti-C and anti-e (5.6%), anti-Leb (4.9%), anti-D (4.6%), anti-Jka (3.1%), anti-S (2.5%), and anti-M (1.9%) were detected. In 41 of the 324 (12.7%) of these patients, the types of antibodies were identified with the NaCl/Enzyme gel test but not with the LISS/Coombs gel test.
CONCLUSIONS
Among the 467 patients, 130 (27.8%) in whom unexpected antibodies were detected, were scheduled for surgery. Because 101 of these 130 patients (77.7%) were unimmunized, unexpected antibody screening may be important in secondary hospitals with patients who do not have a detailed transfusion history. We identified Rh, P, and Lewis group antibodies more efficiently with a combination of the LISS/Coombs gel test and the NaCl/Enzyme gel test.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- The Incidence of Unexpected Antibodies in Transfusion Candidates
- Frequency of Detection of Unexpected Antibodies in Transfusion Candidates at a Secondary Hospital in Gyeongbuk Province
- The Frequency and Distribution of Unexpected Antibodies in Transfusion Candidates with the Use of the Ortho BioVue System: Recent Four Year Experience
- The Frequency of Unexpected Antibodies in Transfusion Candidates in Recent 6 Years
- The frequencies of unexpected antibodies in transfusion candidates and selection of cross-matching method
