Korean J Parasitol.  2012 Jun;50(2):157-159. 10.3347/kjp.2012.50.2.157.

PCR for Diagnosis of Male Trichomonas vaginalis Infection with Chronic Prostatitis and Urethritis

• Lee JJ
• Moon HS
• Lee TY
• Hwang HS
• Ahn MH
• Ryu JS

• KMID: 1384746
• DOI: http://doi.org/10.3347/kjp.2012.50.2.157

Abstract

The aim of this study was to assess the usefulness of PCR for diagnosis of Trichomonas vaginalis infection among male patients with chronic recurrent prostatitis and urethritis. Between June 2001 and December 2003, a total of 33 patients visited the Department of Urology, Hanyang University Guri Hospital and were examined for T. vaginalis infection by PCR and culture in TYM medium. For the PCR, we used primers based on a repetitive sequence cloned from T. vaginalis (TV-E650). Voided bladder urine (VB1 and VB3) was sampled from 33 men with symptoms of lower urinary tract infection (urethral charge, residual urine sensation, and frequency). Culture failed to detect any T. vaginalis infection whereas PCR identified 7 cases of trichomoniasis (21.2%). Five of the 7 cases had been diagnosed with prostatitis and 2 with urethritis. PCR for the 5 prostatitis cases yielded a positive 330 bp band from bothVB1 and VB3, whereas positive results were only obtained from VB1 for the 2 urethritis patients. We showed that the PCR method could detect T. vaginalis when there was only 1 T. vaginalis cell per PCR mixture. Our results strongly support the usefulness of PCR on urine samples for detecting T. vaginalis in chronic prostatitis and urethritis patients.

Keyword

Trichomonas vaginalis; PCR; prostatitis; urethritis; urine

MeSH Terms

Adult
DNA Primers/genetics
Humans
Male
Middle Aged
Molecular Diagnostic Techniques/*methods
Parasitology/*methods
Polymerase Chain Reaction/*methods
Prostatitis/diagnosis/parasitology
Republic of Korea
Trichomonas Infections/*diagnosis/parasitology
Trichomonas vaginalis/genetics/*isolation & purification
Urethritis/diagnosis/parasitology