Korean J Parasitol.  2012 Jun;50(2):99-102. 10.3347/kjp.2012.50.2.99.

IgG Avidity ELISA Test for Diagnosis of Acute Toxoplasmosis in Humans

• Rahbari AH
• Keshavarz
• Shojaee S
• Mohebali M
• Rezaeian M

• KMID: 1384736
• DOI: http://doi.org/10.3347/kjp.2012.50.2.99

Abstract

Serum samples, 100 in the total number, were collected from different laboratories in Tehran, Iran and tested for anti-Toxoplasma specific IgG and IgM antibodies using indirect immunofluorescent antibody test (IFAT). Using the IgG (chronic) and IgM (acute) positive samples, the IgG avidity test was performed by ELISA in duplicate rows of 96-well microtiter plates. One row was washed with 6 M urea and the other with PBS (pH 7.2), then the avidity index (AI) was calculated. Sixteen out of 18 (88.9%) sera with acute toxoplasmosis showed low avidity levels (AI< or =50), and 76 out of 82 (92.7%) sera in chronic phase of infection showed high avidity index (AI>60). Six sera had borderline ranges of AI. The results showed that the IgG avidity test by ELISA could distinguish the acute and chronic stages of toxoplasmosis in humans.

Keyword

Toxoplasma gondii; IgG avidity test; ELISA; IgM; indirect immunofluorescent antibody test

MeSH Terms

Antibodies, Protozoan/*blood
*Antibody Affinity
Clinical Laboratory Techniques/*methods
Enzyme-Linked Immunosorbent Assay/methods
Fluorescent Antibody Technique, Indirect
Humans
Immunoglobulin G/*blood
Immunoglobulin M/blood
Iran
Parasitology/*methods
Toxoplasmosis/*diagnosis