Exp Mol Med.  2004 Apr;36(2):110-115.

Association study of the angiotensin-converting enzyme (ACE) gene G2350A dimorphism with myocardial infarction

• Iqbal MP
• Mahmood S
• Mehboobali N
• Ishaq M
• Fatima T
• Parveen S
• Frossard P

Abstract

The angiotensin converting enzyme (ACE) is a strong candidate gene for myocardial infarction (MI). Insertion-deletion dimorphism in intron 16 of this gene has been inconclusively found to be associated with it. Several new polymorphisms in the ACE gene have been identified and among these, a dimorphism in exon 17, ACE G2350A, has a significant effect on plasma ACE concentrations. To assess the value of genotyping the ACE G2350A dimorphism in a genetically homogeneous population, we carried out a case-control study of dimorphism G2350A for a putative association with MI among Pakistani nationals. We investigated a sample population of 370 Pakistanis, comprising 163 controls, and 207 patients with clinical diagnosis of acute MI (AMI). ACE G2350A alleles were visualized by assays based on polymerase chain reaction and restriction endonuclease analysis. Frequencies of G alleles were 0.68 among controls and 0.72 among AMI patients. The ACE G2350A dimorphism showed no significant association with MI (c2=0.90, 2 df, P=0.64), plasma levels of homocysteine (P=0.52) or with serum levels of folate (P=0.299). The results indicate that ACE G2350A polymorphism is not associated with risk of myocardial infarction in the Pakistani population investigated here.

Keyword

angiotensin converting enzyme; folic acid; genetics; homocysteine; myocardial infarction; Pakistani population

MeSH Terms

Adult
Aged
Exons/*genetics
Female
Genetic Predisposition to Disease
Genetics, Population
Genotype
Humans
Male
Middle Aged
*Mutation
Myocardial Infarction/blood/*genetics
Peptidyl-Dipeptidase A/blood/*genetics
*Polymorphism, Genetic
Predictive Value of Tests