J Vet Sci.  2011 Dec;12(4):401-403. 10.4142/jvs.2011.12.4.401.

Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate

  • 1Department of Infectious Disease, College of Veterinary Medicine and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul 151-742, Korea. yoohs@snu.ac.kr
  • 2Department of Medicinal Plant Resources, Nambu University, Gwangju 506-824, Korea.
  • 3Division of Biological Sciences and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 561-756, Korea.


Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine candidate, were observed to induce Apx-specific immune responses in mice. These results suggest that transgenic corn-derived ApxIIA and CTB-ApxIIA#5 proteins are potential vaccine candidates against A. pleuropneumoniae infection.


Actinobacillus pleuropneumoniae; ApxIIA; cholera toxin B subunit protein; transgenic plant

MeSH Terms

Actinobacillus Infections/microbiology/*prevention & control
Actinobacillus pleuropneumoniae
Antigens, Bacterial/immunology
Bacterial Proteins/*immunology
Bacterial Vaccines/*immunology
Cholera Toxin/*chemistry
Hemolysin Proteins/*immunology
Immunization, Secondary
Mice, Inbred ICR
Plants, Genetically Modified
Zea mays/*genetics
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