J Vet Sci.  2004 Jun;5(2):125-130.

Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea

• Yang DK
• Kim BH
• Kweon CH
• Kwon JH
• Lim SI
• Han HR

Abstract

A virus strain, showing cytopathic effect in Vero cell, was isolated from plasma of a fattening pig in Gyeonggi province, Korea in October 1999. The evaluation of physicochemical/biological properties of the isolate showed that the virus, KV1899, inoculated suckling mouse showed paralysis and died within 7 days post-inoculation, the mouse brain suspension had hemagglutinating activity with goose RBC. Pathogenicity of isolate was carried out by intracranial and intraperitoneal inoculation of 3-4 weeks mice. The mice inoculated with isolate showed 10 4.5 LD50/ 0.03 ml and 10 3.0 LD50/0.5 ml according to the inoculation route. The isolate was identified as RNA and enveloped virus using IUDR and chloroform sensitivity test. The virus particles within the infected Vero cell were measured to be 40-50 nm in size by electron microscopy. The isolate was further characterized by immuno-fluorescence assay using Japanese encephalitis virus (JEV) specific monoclonal antibodies. Reverse transcription polymerase chain reaction (RT-PCR) revealed the presence of JE specific conserved sequences in this isolate. The artificially inoculated pigs had HI titer of 320 to 2,560 against JEV at 14 to 42 days post inoculation. We confirmed this isolate as Japanese encephalitis virus. It was the second isolation of JEV in pigs in Korea.

Keyword

Japanese encephalitis virus; Isolation; Identification

MeSH Terms

Animals
Antibodies, Viral/analysis
Cercopithecus aethiops
Cytopathogenic Effect, Viral
Encephalitis Virus, Japanese/*classification/*isolation & purification/ultrastructure
Encephalitis, Japanese/pathology/*veterinary/virology
Fluorescent Antibody Technique, Indirect/veterinary
Hemagglutination Inhibition Tests/veterinary
Hemagglutination Tests/veterinary
Korea
Mice
Microscopy, Electron/veterinary
RNA, Viral/analysis
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Swine
Swine Diseases/pathology/*virology
Vero Cells/virology