Exp Mol Med.
2003 Dec;35(6):501-508.
High levels of soluble herpes virus entry mediator in sera of patients with allergic and autoimmune diseases
- Affiliations
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- 1The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan 680-749, Korea. bskwon@mail.ulsan.ac.kr
- 2The Immunomics Inc., Ulsan 680-749, Korea.
- 3Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
- 4Department of Oral Microbiology, College of Dentistry, Seoul National University, Seoul 110-744, Korea.
- 5Department of Internal Medicine, Ulsan University Hospital, University of Ulsan, Ulsan 682-714, Korea.
- 6Department of Surgery, Ulsan University Hospital, University of Ulsan, Ulsan 682-714, Korea.
- 7LSU Eye Center, Louisiana State University School of Medicine, 2020 Gravier Street New Orleans LA 70112, USA.
Abstract
- Herpes virus entry mediator (HVEM) is a newly discovered member of the tumor necrosis factor receptor (TNFR) superfamily that has a role in herpes simplex virus entry, in T cell activation and in tumor immunity. We generated mAb against HVEM and detected soluble HVEM (SHVEM) in the sera of patients with various autoimmune diseases. HVEM was constitutively expressed on CD4(+)and CD8(+)T cells, CD19(+)B cells, CD14(+)monocytes, neutrophils and dendritic cells. In three-way MLR, mAb 122 and 139 were agonists and mAb 108 had blocking activity. An ELISA was developed to detect sHVEM in patient sera. sHVEM levels were elevated in sera of patients with allergic asthma, atopic dermatitis and rheumatoid arthritis. The mAbs discussed here may be useful for studies of the role of HVEM in immune responses. Detection of soluble HVEM might have diagnostic and prognostic value in certain immunological disorders.
Keyword
MeSH Terms
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Animals
Antibodies, Monoclonal/immunology
Antibody Specificity
Arthritis, Rheumatoid/blood/immunology
Asthma/blood/immunology
Autoimmune Diseases/*blood/immunology
Cell Division
Cell Line
Dermatitis, Atopic/blood/immunology
Female
Flow Cytometry
Humans
Hypersensitivity/*blood/immunology
Lymphocyte Culture Test, Mixed
Mice
Mice, Inbred BALB C
Receptors, Tumor Necrosis Factor/*blood/immunology
Receptors, Tumor Necrosis Factor, Member 14
Receptors, Virus/*blood/immunology
Solubility
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