J Vet Sci.  2010 Jun;11(2):125-131. 10.4142/jvs.2010.11.2.125.

spa typing and enterotoxin gene profile of Staphylococcus aureus isolated from bovine raw milk in Korea

• Hwang SY
• Park YK
• Koo HC
• Park YH

• KMID: 1110859
• DOI: http://doi.org/10.4142/jvs.2010.11.2.125

Abstract

Staphylococcus aureus is a major etiological pathogen of bovine mastitis, which triggers significant economic losses in dairy herds worldwide. In this study, S. aureus strains isolated from the milk of cows suffering from mastitis in Korea were investigated by spa typing and staphylococcal enterotoxin (SE) gene profiling. Forty-four S. aureus strains were isolated from 26 farms in five provinces. All isolates grouped into five clusters and two singletons based on 14 spa types. Cluster 1 and 2 isolates comprised 38.6% and 36.4% of total isolates, respectively, which were distributed in more than four provinces. SE and SE-like toxin genes were detected in 34 (77.3%) isolates and the most frequently detected SE gene profile was seg, sei, selm, seln, and selo genes (16 isolates, 36.3%), which was comparable to one of the genomic islands, Type I nuSabeta. This is a first report of spa types and the prevalence of the recently described SE and SE-like toxin genes among S. aureus isolates from bovine raw milk in Korea. Two predominant spa groups were distributed widely and recently described SE and SE-like toxin genes were detected frequently.

Keyword

bovine mastitis; enterotoxin; mobile genetic elements; S. aureus; spa typing

MeSH Terms

Animals
Cattle
Cluster Analysis
DNA, Bacterial/chemistry/genetics
Enterotoxins/chemistry/*genetics
Female
Genotype
Mastitis, Bovine/epidemiology/genetics/*microbiology
Microsatellite Repeats
Milk/*microbiology
Polymerase Chain Reaction/veterinary
Prevalence
Republic of Korea/epidemiology
Staphylococcal Infections/epidemiology/microbiology/*veterinary
Staphylococcus aureus/classification/genetics/*isolation & purification

Figure

• 

  Figure 1 The number of screened herds and collected samples from five provinces of South Korea. The detection rates of S. aureus are shown in boxes and arrows denote each province.

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