Exp Mol Med.  2006 Aug;38(4):445-452.

Inhibitory effects of tilianin on the expression of inducible nitric oxide synthase in low density lipoprotein receptor deficiency mice

• Nam KH
• Choi JH
• Seo YJ
• Lee YM
• Won YS
• Lee MR
• Lee MN
• Park JG
• Kim YM
• Kim HC
• Lee CH
• Lee HK
• Oh SR
• Oh GT

Abstract

We investigated the effect of tilianin upon inducible nitric oxide synthesis in the plasma of low-density lipoprotein receptor knock-out (Ldlr-/-) mice fed with high cholesterol diet and in primary peritoneal macrophages of Ldlr-/- mice. High cholesterol diet induced nitric oxide production in the plasma of Ldlr-/- mice. Tilianin reduced the level of nitric oxide (NO) in plasma from Ldlr-/- mice induced by the high cholesterol diet. Tilianin also inhibited the NO production from the primary culture of peritoneal macrophages treated with lipopolysaccharide. The inhibition of NO production was caused by the suppression of inducible nitric oxide synthase (iNOS) gene expression in peritoneal macrophages isolated from Ldlr-/- mice. Moreover, tilianin inhibited the transcriptional activation of iNOS promoter that has NF-kappa B binding element. Thus, these results provide the first evidence that tilianin inhibit iNOS expression and production of NO and may act as a potential anti-inflammatory agent.

Keyword

anti-inflammatory agents; hyperlipoproteinemia; nitric oxide synthase type II; nitric oxide; tilianin

MeSH Terms

Tyrosine/analogs & derivatives/metabolism
Tissue Distribution
Sinus of Valsalva/metabolism/pathology/ultrastructure
Receptors, LDL/*genetics
Promoter Regions (Genetics)/drug effects
Nitric Oxide Synthase Type II/*metabolism
Nitric Oxide/biosynthesis/blood
NF-kappa B/metabolism
Mice, Knockout
Mice
Male
Inflammation/metabolism
Glycosides/*pharmacology
Flavonoids/*pharmacology
Down-Regulation/drug effects
Atherosclerosis/metabolism
Animals