Exp Mol Med.  1998 Sep;30(3):137-144.

Role of Ca2+ influx in the tert-butyl hydroperoxide-induced apoptosis of HepG2 human hepatoblastoma cellse

• Kim JA
• Kang YS
• Kim YO
• Lee SH
• Lee YS

Abstract

Oxidative stress appears to be implicated in the pathogenesis of various diseases including alcoholic liver injury. In this study we investigated the mechanism of apoptosis induced by tert-butyl hydroperoxide (TBHP) in HepG2 human hepatoblastoma cells. Treatment with TBHP significantly reduced glutathione content and glutathione reductase activity, and increased glutathione peroxidase activity, indicating that TBHP induced oxidative stress in the HepG2 cells. TBHP also induced reduction of cell viability and DNA fragmentation, a hallmark of apoptosis, in a dose-dependent manner. In addition, TBHP induced a sustained increase in intracellular Ca2+ concentration, which was completely prevented by the extracellular Ca2+ chelation with EGTA. TBHP also induced Mn2+ influx. These
results
indicate that the intracellular Ca2+ increase by TBHP is exclusively due to Ca2+ influx from the extracellular site. Treatment with either an extracellular (EGTA) or an intracellular Ca2+ chelator (BAPTA/AM) significantly suppressed the TBHP-induced apoptosis. Taken together, these results suggest that TBHP induced the apoptotic cell death in the HepG2 cells and that Ca2+ influx may play an important role in the apoptosis induced by TBHP.

Keyword

tert-butyl hydroperoxide; oxidative stress; apoptosis; HepG2 cells; intracellular Ca2+; Mn2+ influx

MeSH Terms

Apoptosis/drug effects*
Calcium Signaling/drug effects*
Chelating Agents/pharmacology
Egtazic Acid/pharmacology
Egtazic Acid/analogs & derivatives
Hepatoblastoma/pathology*
Hepatoblastoma/metabolism
Hepatoblastoma/drug therapy
Human
Manganese/metabolism
Oxidative Stress/drug effects
Tumor Cells, Cultured
tert-Butylhydroperoxide/pharmacology*