J Vet Sci.  2001 Aug;2(2):115-120.

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

• Shin IS
• Kim HR
• Nam MJ
• Youn HY

Abstract

This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and rat IL-6, respectively.

Keyword

Cytokine; cDNA; cloning; PCR; IL-3; IL-6; dog

MeSH Terms

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Concanavalin A/pharmacology
DNA, Complementary/*chemistry
Dogs/blood/genetics/*immunology
Interleukin-3/chemistry/*genetics
Interleukin-6/chemistry/*genetics
Leukocytes, Mononuclear/chemistry/drug effects
Molecular Sequence Data
Open Reading Frames/genetics
Protein Sorting Signals/genetics
RNA/blood/genetics
Reverse Transcriptase Polymerase Chain Reaction/veterinary
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid