J Vet Sci.  2009 Jun;10(2):141-146. 10.4142/jvs.2009.10.2.141.

NaCl plus chitosan as a dietary salt to prevent the development of hypertension in spontaneously hypertensive rats

• Park SH
• Dutta NK
• Baek MW
• Kim DJ
• Na YR
• Seok SH
• Lee BH
• Cho JE
• Cho GS
• Park JH

• KMID: 1102970
• DOI: http://doi.org/10.4142/jvs.2009.10.2.141

Abstract

The effect of NaCl plus 3% chitosan on the systolic blood pressure of spontaneously hypertensive rats (SHR) were evaluated and compared with NaCl plus KCl (NaCl, 49.36% + KCl 49.36%) and chitosan or NaCl treatment alone. In SHR, administration of NaCl plus chitosan (44 mM Na/day) for two months significantly decreased the systolic blood pressure greater than of NaCl plus KCl and NaCl alone. NaCl plus chitosan resulted, though not statistically significant, in decreased urinary Na+ excretion and decreased blood urea nitrogen levels. Urinary creatinine of NaCl plus chitosan was slightly decreased compared to 3 treated groups. Serum electrolytes levels, however, remained unchanged. The combination of NaCl and chitosan may be superior to the conventional use of NaCl plus KCl or NaCl alone in the prevention of hypertension. Even though these supplementary diets have demonstrated potential anti-hypertensive effects in the experimental animal model, further research is needed before any recommendations can be made.

Keyword

chitosan; hypertension; KCl; NaCl; spontaneously hypertensive rat

MeSH Terms

Angiotensin I/blood
Angiotensin II/biosynthesis
Animals
Blood Pressure/*drug effects/physiology
Blood Urea Nitrogen
Body Weight/drug effects
Chitosan/*administration & dosage
Chlorides/blood/urine
Creatinine/urine
Heart/physiology
Histocytochemistry
Hypertension/*prevention & control
Kidney/physiology
Male
Potassium/blood/urine
Potassium Chloride/administration & dosage
Random Allocation
Rats
Rats, Inbred SHR
Sodium/blood/urine
Sodium Chloride, Dietary/*administration & dosage
Systole/drug effects/physiology

Figure

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  Fig. 1 Changes in body weight of spontaneously hypertensive rats administered dietary salts over the experimental period. Vertical bars represent the mean ± SD (n = 5).

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  Fig. 2 Changes in systolic blood pressure of spontaneously hypertensive rats administered various combinations of dietary salts. Means with the same alphabetical letter are not significantly different (p < 0.05). Vertical bars represent the mean ± SD (n = 5).

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  Fig. 3 Effect of dietary salts on serum angiotensin 1 and 2 concentrations (3A and 3B). Vertical bars represent the mean ± SD (n = 5).

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  Fig. 4 Effect of dietary salts on serum electrolytes (Na+, K+ and Cl-). Vertical bars represent the mean ± SD (n = 5).

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  Fig. 5 Effect of dietary salts on urine electrolytes (Na+, K+ and Cl-). Vertical bars represent the mean ± SD (n = 5).

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  Fig. 6 Influence of different diets on urine blood urea nitrogen and creatinine levels in spontaneously hypertensive rats. Means with the different alphabetical letter are significantly different (p < 0.05) compared with the control group.

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