J Vet Sci.  2008 Mar;9(1):85-90. 10.4142/jvs.2008.9.1.85.

Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea

• Joh SJ
• Shon CI
• Kang SW
• Kim BH
• Jeong BY
• Lee KG
• Kwon JH
• Heo GJ

• KMID: 1102957
• DOI: http://doi.org/10.4142/jvs.2008.9.1.85

Abstract

The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1 contains the Leu-Lys-Asn (LKN) motif instead of the typical Gly-Asp- Asp (GDD) motif found in other aquatic birnaviruses. We also identified the GLPYIGKT motif, the putative GTPbinding site at amino acid position 248. In total, the VP1 regions of 22 birnavirus strains were compared for analyzing the genetic relationship among the family Birnaviridae. Based on the deduced amino acid sequences, GC1 was observed to be more closely related to the infectious pancreatic necrosis virus (IPNV) from the USA, Japan, and Korea than the IPNV from Europe. Further, aquatic birnaviruses containing GC1 and IPNV have genogroups that are distinct from those in the genus Avibirnaviruses and Entomo-birnaviruses. The birnavirusstrains were clustered into 5 genogroups based on their amino acid sequences. The marine aquatic birnaviruses (MABVs) containing GC1 were included in the MABV genogroup; the IPNV strains isolated from Korea, Japan, and the USA were included in genogroup 1 and the IPNV strains isolated primarily from Europe were included in genogroup 2. Avibirnaviruses and entomobirnaviruses were included in genogroup 3 and 4, respectively.

Keyword

aquatic birnavirus; GC1; genetic characterization; rockfish Sebastes schlegeli; VP1

MeSH Terms

Amino Acid Sequence
Animals
Base Sequence
Birnaviridae/classification/*genetics
Capsid Proteins/chemistry/*genetics
Cell Line
Fishes/*virology
Korea
Molecular Sequence Data
Phylogeny

Figure

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  Fig. 1 Cladogram representing phylogenetic relationships between birnaviruses based on deduced amino acid sequences of VP1. The length of each pair of branches represents the distance between the sequence pairs, and the numbers in parentheses indicate the bootstrap values.

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