Korean J Intern Med.  2008 Dec;23(4):165-169. 10.3904/kjim.2008.23.4.165.

Hepatocyte nuclear factor 1-alpha mutation in normal glucose-tolerant subjects and early-onset type 2 diabetic patients

• Lim DM
• Huh N
• Park KY

• KMID: 1100763
• DOI: http://doi.org/10.3904/kjim.2008.23.4.165

Abstract

BACKGROUND/AIMS: The prevalence of diabetes in Korea is reported to be approximately 10%, but cases of maturity-onset diabetes of the young (MODY) are rare in Korea. A diagnostic technique for autosomal dominant MODY is being actively sought. In this regard, we used a DNA chip to investigate the frequency of mutations of the MODY3 gene (hepatocyte nuclear factor-1alpha) in Korean patients with early-onset type 2 diabetes. METHODS: The genomic DNA of 30 normal individuals [age, 24.9+/-8.6 years] and 25 patients with early-onset type 2 diabetes (age, 27+/-5.9 years) was extracted, and the MODY3 gene was amplified. The amplified DNA was hybridized onto a MODY3 chip, which has oligonucleotides of 15-25 bases, representing wild-type and mutant MODY3 sequences in both forward and reverse orientations, immobilized on its surface. RESULTS: Among the normal subjects, there was no mutation of MODY3. Among those with early-onset type 2 diabetes, there was one case of MODY3 mutation. CONCLUSIONS: Our results indicate that MODY3 mutations are not rare in Korean early-onset type 2 diabetes patients in Korea and suggest that MODY3 mutations in patients with early-onset type 2 diabetes need to be further evaluated.

Keyword

DNA chips; MODY; Type 2 diabetes mellitus

MeSH Terms

Adolescent
Adult
Age of Onset
Asian Continental Ancestry Group/genetics/*statistics & numerical data
Case-Control Studies
Cohort Studies
Diabetes Mellitus, Type 2/*epidemiology/*genetics
Female
Hepatocyte Nuclear Factor 1-alpha/*genetics
Humans
Korea
Male
Mutation/*genetics
Prevalence
Young Adult