Korean J Parasitol.  2011 Jun;49(2):133-138. 10.3347/kjp.2011.49.2.133.

Hematological and Serum Biochemical Analyses in Experimental Caprine Besnoitiosis

• Nazifi S
• Oryan A
• Namazi F

• KMID: 1090612
• DOI: http://doi.org/10.3347/kjp.2011.49.2.133

Abstract

This study was undertaken to investigate the hematological and biochemical changes in experimentally infected goats with Besnoitia caprae from the time of infection till 360 days post-infection (PI). Six male goats were inoculated subcutaneously with 13x10(7) bradyzoites of B. caprae, and blood samples were collected from the jugular vein. The total erythrocyte and total leukocyte counts, hematocrit value, and differential leukocyte counts were determined. Serum biochemical analysis, including the total protein, albumin, total globulin, cholesterol, triglyceride, chloride, testosterone, calcium (Ca2+), inorganic phosphorus, sodium (Na+), potassium (K+), iron (Fe2+), glucose, serum amyloid A (SAA), haptoglobin (Hp), fibrinogen, ceruloplasmin, aspartate aminotransferase, alanine aminotransferase, creatine kinase, lactate dehydrogenase, and alkaline phosphatase, was undertaken. Skin biopsy from the limbs were collected at weekly intervals and histologically examined for Besnoitia cysts. Cysts were present in the skin biopsies of the leg of the infected goats from day 28 PI. There were variations in hematological analyses, but no significant difference was seen. From day 30 to 360 PI, results showed that SAA, Hp, fibrinogen, and ceruloplasmin concentrations increased, whereas testosterone concentrations decreased. Infected goats exhibited decrease of albumin and increase of serum total protein and globulin concentrations. By contrast, there were no significant differences in the remained analyses concentrations.

Keyword

Besnoitia caprae; hematological parameter; serum biochemical analysis; goat

MeSH Terms

Animals
Biopsy
Blood Chemical Analysis
Coccidiosis/*parasitology/*pathology
Disease Models, Animal
Erythrocyte Count
Goat Diseases/*parasitology/*pathology
Goats
Hematocrit
Histocytochemistry
Leukocyte Count
Male
Sarcocystidae/*isolation & purification/*pathogenicity
Skin/pathology
Time Factors