J Vet Sci.  2006 Jun;7(2):189-192. 10.4142/jvs.2006.7.2.189.

Matricaria chamomilla CH12 decreases handling stress in Nelore calves

• Reis LS
• Pardo PE
• Oba E
• Kronka SN
• Frazatti-Gallina NM

• KMID: 1059207
• DOI: http://doi.org/10.4142/jvs.2006.7.2.189

Abstract

Matricaria chamomilla CH12 is a phytotherapeutic or homeopathic product, which has been used to reduce stress. Here, we examined its effect on preventing handling stress in bovines. Sixty Nelore calves were randomly distributed into two equal groups. One group was administered Matricaria chamomilla CH12 in diet and the other the 'control' was not. Animals in both groups were maintained unstressed for 30 days to adjust to the feeding system and pasture, and were then stressed by constraint on the 31th, 38th, 45th and 60th experimental days. Blood samples were taken on these days after animals had been immobilization in a trunk contention for 5 min. Stress was followed by analyzing serum cortisol levels. These peaked on the 45th day and then decreased, but not to baseline, on the 60th day. On the 45th day cortisol levels were significantly lower in animals fed Matricaria chamomilla CH12, suggesting that this product reduces stress. These effects may be a consequence of its inhibiting cortisol production and its calming and anxiolytic effects.

Keyword

bovine; Matricaria chamomilla; cortisol; handling; Nelore calf; stress

MeSH Terms

Animal Feed
Animals
Cattle
Cattle Diseases/*drug therapy
Diet
Matricaria/*chemistry
Plant Preparations/chemistry/*pharmacology
Stress/drug therapy/*veterinary

Figure

• 

  Fig. 1 Effect of Matricaria Chamomilla CH12 on calf serum cortisol. Mean cortisol levels (±SD) of treated and control groups (30 animals/group). Means with the dotted box are statistically different from each other (p < 0.05). Means with at least common lower case letter are similar within the given group.

Reference

1. Amirghofran Z, Azadbakht M, Karimi MH. Evaluation of the immunomodulatory effects of five herbal plants. J Ethnopharmacol. 2000. 72:167–172.

2. Aragón VEF, Graca DS, Norte AL, Santiago GS, Paula OJ. Suplementação com cromo e desempenho reprodutivo de vacas zebu primíparas mantidas a pasto. Arq Bras Med Vet Zootec. 2001. 53:624–628.

3. Avallone R, Zanoli P, Puia G, Kleinschnitz M, Schreier P, Baraldi M. Pharmacological profile of apigenin, a flavonoid isolated from Matricaria chamomilla. Biochem Pharmacol. 2000. 59:1387–1394.

4. Banzatto DA, Kronka SN. Experimentação agrícola. 1995. 3rd ed. Jaboticabal: FUNEP;247.

5. Borell EH. The biology of stress and its application to livestock housing and transportation assessment. J Anim Sci. 2001. 79:E. Suppl.E260–E267.

6. Cook CJ, Mellor DJ, Harris PJ, Ingram JR, Matthews LR. Morberg GP, Mench JA, editors. Hands-on and hands-off measurement of stress. The Biology of Animals Stress: Basic Principles And Implications for Animal Welfare. 2000. New York: CABI Publishing;123–146.

7. Coppo JA, Mussart NB, Revidatti MA, Capellari A. Absence of biochemically demonstrable stress in early weaned half-bred zebu calves. Cien e Inv Agr. 2003. 30:97–105.

8. Dickerson SS, Kemeny ME. Acute stressors and cortisol responses: a theoretical integration and synthesis of laboratory research. Psychol Bull. 2004. 130:355–391.

9. Eicher SD. Transportation of cattle in the dairy industry: current research and future directions. J Anim Sci. 2001. 84:E. Suppl.E19–E23.

10. Elsasser TH, Klasing KC, Filipov N, Thompson F. Morberg GP, Mench JA, editors. The metabolic consequences of stress: Targets for stress and proprieties of nutrient use. The Biology of Animals Stress: Basic Principles And Implications for Animal Welfare. 2000. New York: CABI Publishing;77–100.

11. Encarnação RO. Costa MJRP, editor. Estresse e produção animal. 1° Ciclo internacional de palestras sobre bioclimatologia animal. 1989. Jaboticabal: FUNEP;111–129.

12. Garcia-Belenguer S, Palacio J, Gascón M, Aceña C, Revilla R, Mormède P. Differences in the biological stress responses of two cattle breeds to walking up to mountain pastures in the Pyrenees. Vet Res. 1996. 27:515–526.

13. Goutman JD, Waxemberg MD, Doñate-Oliver F, Pomata PE, Calvo DJ. Flavonoid modulation of ionic currants mediated by GABA_A and GABA_C receptors. Eur J Pharmacol. 2003. 461:79–87.

14. Grandin T. Evaluación del estrés durante el manejo y transporte. J Anim Sci. 1997. 75:249–257.

15. Grandin T. Handling methods and facilities to reduce stress on cattle. Vet Clin North Am Food Anim Pract. 1998. 14:325–342.

16. Hemsworth PH, Coleman GJ, Barnett JL, Borg S. Relationships between human-animal interactions and productivity of commercial dairy cows. J Anim Sci. 2000. 78:2821–2831.

17. Hemsworth PH, Coleman GJ, Barnett JL, Borg S, Dowling S. The effects of cognitive behavioral intervention on the attitude and behavior of stockpersons and the behavior and productivity of commercial dairy cows. J Anim Sci. 2002. 80:68–78.

18. Hickey MC, Drennan M, Earley B. The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocytes, acute-phase proteins and in vitro interferon-gamma production. J Anim Sci. 2003. 81:2847–2855.

19. Hopster H, van der Werf JTN, Erkens JHF, Blokhuis HJ. Effects of repeated jugular puncture on plasma cortisol concentrations in loose-housed dairy cows. J Anim Sci. 1999. 77:708–714.

20. Joca SRL, Padovan CM, Guimarães FS. Estresse, depressão e hipocampo. Rev Bras Psiquiatr. 2003. 25:Suppl. 2. 46–51.

21. Knowles TG, Warriss PD. Grandin T, editor. Stress physiology during transport. Livestock Handling and Transport. 2000. 2nd ed. Wallingford: CAB International;385–407.

22. Kurosawa M, Nagata S, Takeda F, Mima K, Hiraga A, Kai M, Taya K. Plasma catecholamine, adrenocorticotropin and cortisol responses to exhaustive incremental treadmill exercise of the Thoroughbred horse. J Equi Sci. 1998. 9:9–18.

23. Lensink BJ, Fernandez X, Boivin X, Pradel P, Le Neindre P, Veissier I. The impact of gentle contacts on ease of handling, welfare, and growth of calves and on quality of veal meat. J Anim Sci. 2000. 78:1219–1226.

24. Marder M, Paladini AC. GABA(A)-receptor ligands of flavonoid structure. Curr Top Med Chem. 2002. 2:853–867.

25. Marder M, Viola H, Wasowski C, Fernández S, Medina JH, Paladini AC. 6-methylapigenin and hesperidin: new valeriana flavonoids with activity on the CNS. Pharmacol Biochem Behav. 2003. 75:537–545.

26. Matteri RL, Carroll JA, Dyer CJ. Morberg GP, Mench JA, editors. Neuroendocrine responses to stress. The Biology of Animals Stress: Basic Principles and Implications for Animal Welfare. 2000. New York: CABI Publishing;43–76.

27. Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Calvo D, Paladini AC. new ligands for the benzodiazepine receptors. Phytomedicine. 1998. 5:235–243.

28. Mellor DJ, Stafford KJ, Todd SE, Lowe TE, Gregory NG, Bruce RA, Ward RN. A comparison of catecholamine and cortisol responses of young lambs and calves to painful husbandry procedures. Aust Vet J. 2002. 80:228–233.

29. Morrow CJ, Kolver ES, Verkerk GA, Matthews LR. Fecal glucocorticoid metabolites as a measure of adrenal activity in dairy cattle. Gen Comp Endocrinol. 2002. 126:229–241.

30. Nockels CF, Odde KG, Craig AM. Vitamin E supplementation and stress affect tissue á-tocopherol content of beef heifers. J Anim Sci. 1996. 74:672–677.

31. Ohno S, Shinoda S, Toyoshima S, Nakazawa H, Makino T, Nakajin S. Effects of flavonoid phytochemicals on cortisol production and on activities of steroidogenic enzymes in human adrenocortical H295R cells. J Steroid Biochem Mol Biol. 2002. 80:355–363.

32. Pajor EA, Rushen J, de Passile AMB. Aversion learning techniques to evaluate dairy cattle handling practices. Appl Anim Behav Sci. 2000. 69:89–102.

33. Paladini AC, Marder M, Viola H, Wolfman C, Wasowski C, Medina JH. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol. 1999. 51:519–526.

34. Paludo GR, McManus C, Melo RQ, Cardoso AG, Mello FPS, Moreira M, Fuck BH. Efeito do estresse térmico e do exercício sobre pârametros fisiológicos de cavalos do exército brasileiro. R Bras Zootec. 2002. 31:1130–1142.

35. Sánchez JM, Castro MJ, Alonso ME, Gaudioso VR. Adaptive metabolic responses in females of the fighting breed submitted to different sequences of stress stimuli. Physiol Behav. 1996. 60:1047–1052.

36. Solano J, Galindo F, Qrihuela A, Galina CS. The effect of social rank on the physiological response during repeated stressful handling in Zebu cattle (Bos indicus). Physiol Behav. 2004. 82:679–683.

37. Vásquez EFA, Herrera APN. Concentração plasmática de cortisol, uréia, cálcio e fósforo em vacas de corte mantidas a pasto suplementadas com levedura de cromo durante a estação de monta. Ciência Rural (Santa Maria). 2003. 33:743–747.

38. Veissier I, Reenen CG, Andanson S, Leushuis IE. Adrenocorticotropic hormone and cortisol in calves after corticotropin-releasing hormone. J Anim Sci. 1999. 77:2047–2053.

39. Viola H, Wasowski C, Levi de Stein M, Wolfman C, Silveira R, Dajas F, Medina JH, Paladini AC. Apigenin, a component of Matricaria recutita flowers, is a central benzodiazepine receptors-ligand with anxiolytic effects. Planta Med. 1995. 61:213–216.

40. Wasowski C, Marder M, Viola H, Medina JH, Paladini AC. Isolation and identification of 6-methylapigenin, a competitive ligand for the brain GABA_A receptors, from Valeriana wallichii. Planta Med. 2002. 68:934–936.

41. Yamada K, Miura T, Mimaki Y, Sashida Y. Effect of inhalation of Chamomilla oil vapour on plasma ACTH level in ovariectomized-rat under restriction stress. Biol Pharm Bull. 1996. 19:1244–1246.

42. Zanoli P, Avaloni R, Baraldi M. Behavioral characterization of the flavonoids apigenin and chrysin. Fitoterapia. 2000. 71:Suppl. 1. S117–S123.