J Vet Sci.  2006 Jun;7(2):177-180. 10.4142/jvs.2006.7.2.177.

Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry

• Shitandi A
• Oketch A
• Mahungu S

• KMID: 1059205
• DOI: http://doi.org/10.4142/jvs.2006.7.2.177

Abstract

A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specically sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95% of the test results were interpreted as positive. The limits of detection in liver tissue were 0.35 microgram/ml for furazolidone, 0.70 microgram/ml for sulfamethazine and 7.80 microgram/ ml for amprolium. In kidney tissues, they were 0.30 microgram/ml for furazolidone, 0.54 microgram/ml for sulfamethazine, and 7.6 microgram/ml for amprolium. It was concluded that this tube test could be used to screen for the residue of these three drugs in poultry.

Keyword

anticoccidial drug; Bacillus stearothermophilus; detection limit; poultry tissues

MeSH Terms

Amprolium/analysis
Animals
Bacillus stearothermophilus/*drug effects
Coccidiostats/*analysis
Drug Residues/*analysis
Furazolidone/analysis
Kidney/chemistry
Liver/chemistry
*Poultry
Sulfamethazine/analysis

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