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Towards developing a diagnostic regimen for the treatment follow-up of Trypanosoma brucei gambiense

Mbati PA, Hirumi K, Inoue N, Situakibanza NH, Hirumi H

BALB/c mice infected with a high virulent strain of Trypanosoma brucei gambiense IL3707 were treated intraperitoneally (ip) with either Melarsoprol (Mel-B) or PSG(+) buffer as controls. The mice were subsequently...
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Changes of splenocyte IFN-gamma mRNA synthesis in rats infected with Paragonimus westermani

Cho JK, Kwon HS, Joo KH, Lee JS, Cho SW

Changes in the expression level of splenocyte IFN-gamma mRNA of Sprague-Dawley (SD) rats infected with Paragonimus westermani were analyzed by competitive reverse transcription-polymerase chain reaction (RT-PCR) followed by southern...
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Expression of major piroplasm protein (p33) of Theileria sergenti (Korean isolate) and its immunogenicity in guinea pigs

Kang SW, Kweon CH, Choi EJ, Yoon YD

To investigate the development of a subunit vaccine against theileriosis in cattle, the DNA fragments encoding piroplasm surface protein (p33) of Theileria sergenti of a Korean isolate were expressed...
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Karyotypes of Pneumocystis carinii derived from several mammals

Cho SR, Park YG, Moon HN, Lee SH, Hong ST

Pneumocystis carinii is the most important opportunistic pathogen of humans in the world. Pneumocystis carinii is experimentally detected in the lungs of rats, mice, rabbits, and monkeys, however, the...
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Two new genotypes of Plasmodium vivax circumsporozoite protein found in the Republic of Korea

Kho WG, Park YH, Chung JY, Kim JP, Hong ST, Lee WJ, Kim TS, Lee JS

The gene encoding Plasmodium vivax circumsporozoite protein (PvCSP) exhibits polymorphism in many geographical isolates. The present study was designed to investigate polymorphism in PvCSP gene of P. vivax isolates...
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Upregulated expression of the cDNA fragment possibly related to the virulence of Acanthamoeba culbertsoni

Im KI, Park KM, Yong TS, Hong YP, Kim TE

Identification of the genes responsible for the recovery of virulence in brain-passaged Acanthamoeba culbertsoni was attempted via mRNA differential display-polymerase chain reaction (mRNA DD-PCR) analysis. In order to identify...
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Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA

Sohn WM, Nam HW

A total of 198 sera from stray cats was assayed against Toxoplasma gondii antigen by western blot. Out of 198 sera assayed, 26 sera (13.1%) showed typical blot patterns...
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Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis

Hong ST, Lee M, Sung NJ, Cho SR, Chai JY, Lee SH

The present study analyzed serum IgG subclass antibody reaction to major antigenic bands of Clonorchis sinensis to investigate improvement of its serodiagnosis. Of the four subclass antibodies, IgG1 and...
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A cytogenetic study on human intestinal trematodes of the genus Metagonimus (Digenea: Heterophyidae) in Korea

Lee SU, Huh S, Park GM, Chai JY

In order to analyze chromosome numbers and karyotypes of intestinal trematodes belonging to the genus, Metagonimus, the gonad tissues of M. takahashii, M. miyatai, and M. yokogawai were prepared...
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Isoenzyme patterns and phylogenetic relationships in Acanthamoeba spp. isolated from contact lens containers in Korea

Shin HJ, Cho MS, Kim HJ, Im KI

In order to refer to the basic information regarding the identification of isolates obtained from a contact lens container in Korea, the isoelectric focusing gel electrophoresis was employed to...
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General properties and phylogenetic utilities of nuclear ribosomal DNA and mitochondrial DNA commonly used in molecular systematics

Hwang UW, Kim W

To choose one or more appropriate molecular markers or gene regions for resolving a particular systematic question among the organisms at a certain categorical level is still a very...
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Phylogeny, host-parasite relationship and zoogeography

Hasegawa H

Phylogeny is the evolutionary history of a group or the lineage of organisms and is reconstructed based on morphological, molecular and other characteristics. The genealogical relationship of a group...
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