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CD70 Expression on Spodoptera Frugiperda(Sf9) Cells by Baculovirus to Induce CD27 Stimulation in Mouse B Cells

Woo SY

No abstract available in English.
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Virulence of Entomopathogenic Fungi Metarhizium anisopliae and Paecilomyces fumosoroseus for the Microbial Control of Spodoptera exigua

Han JH, Jin BR, Kim JJ, Lee SY

The beet armyworm Spodoptera exigua (Lepidoptera: Noctuidae) is difficult to control using chemical insecticides because of the development of insecticide resistance. Several pest control agents are used to control the...
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Evaluation of Strains of Metarhizium anisopliae and Beauveria bassiana against Spodoptera litura on the Basis of Their Virulence, Germination Rate, Conidia Production, Radial Growth and Enzyme Activity

Petlamul W, Prasertsan P

Ten strains of the entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana were evaluated to find the most effective strain for optimization studies. The first criterion tested for strain selection was...
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Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

Gao J, Meng C, Chen Z, Li C, Liu G

Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit...
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Insecticidal Activity of Ethyl Acetate Extracts from Culture Filtrates of Mangrove Fungal Endophytes

Abraham S, Basukriadi A, Pawiroharsono S, Sjamsuridzal W

In the search for novel potent fungi-derived bioactive compounds for bioinsecticide applications, crude ethyl acetate culture filtrate extracts from 110 mangrove fungal endophytes were screened for their toxicity. Toxicity tests...
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Expression of Green Fluorescent Protein in Both Spodoptera frugiperda Cells and Bombyx mori Larvae by Ac-Bm Hybrid Virus

Jin BR, Yoon HJ, Yun EY, Kang SW, Cho ES, Kang SK

  • KMID: 1551989
  • J Korean Soc Virol.
  • 1998 Sep;28(3):225-232.
We have expressed GFP in Sf9 and Bm5 cells or Bombyx by larvae by using Ac-Bm hybrid virus capable of replicating in both Bm5 and Sf9 cells. Genomic DNA of...
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Expression of Bovine Growth Hormone Gene in a Baculovirus, Hyphantria cunea Nuclear Polyhedrosis Virus

Park KJ, Lee KK, Kang BJ, Cha SC, Lee HH

  • KMID: 1551974
  • J Korean Soc Virol.
  • 1998 Jun;28(2):129-138.
Bovine growth hormone (bGH) gene was expressed in an insect spodoptera frugiperda cell line using a Baculovirus, Hyphantria cunea nuclear polyhedrosis virus (HcNPV). The bGH gene in pbGH plasmid was...
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Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Choi KS, Kye SJ, Jeon WJ, Park MJ, Kim S, Seul HJ, Kwon JH

A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from...
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Inactivated genotype 1 Japanese encephalitis vaccine for swine

Yang DK, Nah JJ, Kim HH, Song JY

PURPOSE: Japanese encephalitis is a reproductive disorder caused by Japanese encephalitis virus (JEV) in swine. Recent genotype (G) shift phenomenon (G3 to G1) in the Asia-wide has posed a challenge...
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Biological Property of Recombinant Hemagglutinin-Neuraminidase Protein of Avian Paramyxovirus Type 6 Expressed by Recombinant Baculovirus

Kim JY, Lee HJ, Kye SJ, Kim S, Seul HJ, Kim SE, Lee HS, Jung SC, Choi KS

Hemagglutination inhibition (HI) test employing whole virus antigen is a prescribed serological test for serotyping, diagnosis and surveillance for avian paramyxoviruses (APMVs). For use as alternative to the virus antigen,...
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Detection and Quantification of Residual Cellular DNA in the Production of Recombinant HPV-16 L1 Virus-Like Particles

Son HK, Jeong HS, Kim YL, Kim SN, Shin JH, Min HK, Seong BL, Park SN

A number of recombinant proteins isolated from cell sources are being produced for biopharmaceuticals. Although most biopharmaceuticals are highly purified, there is a safety concern that such recombinant products could...
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Biochemical properties of full-length hepatitis C virus RNA-dependent RNA polymerase expressed in insect cells

Choi HB, Kim YG, Oh JW

  • KMID: 1119328
  • Exp Mol Med.
  • 2003 Dec;35(6):475-485.
The hepatitis C virus (HCV) RNA-dependent RNA polymerase, NS5B protein, is the key viral enzyme responsible for replication of the HCV viral RNA genome. Although several full-length and truncated forms...
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Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutininneuraminidase proteins

Lee YJ, Sung HW, Choi JG, Lee EK, Yoon H, Kim JH, Song CS

Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the...
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Synthesis of recombinant blood coagulation factor VIII (FVIII) heavy and light chains and reconstitution of active form of FVIII

Oh SH, Lee MY, Song DW

  • KMID: 1029938
  • Exp Mol Med.
  • 1999 Jun;31(2):95-100.
FVIII is synthesized as a single chain precursor of approximately 280 kD with the domain structure of A1-A2-B-A3-C1-C2 and it circulates as a series of metal ion-linked heterodimers that result from cleavages...
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