- Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry
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Siddiki AM
- KMID: 1705511
- J Vet Sci.
- 2013 Jun;14(2):107-114.
- doi: 10.4142/jvs.2013.14.2.107
Despite the development of new technologies, new challenges still remain for large scale proteomic profiling when dealing with complex biological mixtures. Fractionation prior to liquid chromatography tandem mass spectrometry (LC-MS/MS)... -
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- Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites
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Lee EG, Kim JH, Shin YS, Shin GW, Kim YH, Kim GS, Kim DY, Jung TS, Suh MD
- KMID: 1120070
- J Vet Sci.
- 2004 Jun;5(2):139-145.
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the... -
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- Determination of antigenic domain in GST fused major surface protein (Nc-p43) of Neospora caninum
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Son ES, Ahn HJ, Kim JH, Kim DY, Nam HW
- KMID: 1039175
- Korean J Parasitol.
- 2001 Sep;39(3):241-246.
- doi: 10.3347/kjp.2001.39.3.241
The antigenic domain of the major surface protein (Nc-p43) of Neospora caninum was examined by polymerase chain reaction of its gene fragments and recombinant expression as GST fusion proteins. The... -
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