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Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

Kim HK, Lyoo KS, Huynh TM, Moon HJ, Nguyen VG, Park BK

There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse...
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Development of a novel diagnostic test for detection of bovine viral diarrhea persistently infected animals using hair

Singh K, Miller MM, Kohrt LJ, Scherba G, Garrett EF, Fredrickson RL

The purpose of this study was to determine whether manually plucked hairs might serve as an alternative sample for a quantitative real time polymerase chain reaction (qRT-PCR) testing. Twenty three,...
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TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus

Yang DK, Kweon CH, Kim BH, Lim SI, Kim SH, Kwon JH, Han HR

  • KMID: 1093630
  • J Vet Sci.
  • 2004 Dec;5(4):345-351.
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the...
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Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries

Onuk EE, Ciftci A, Findik A, Durmaz Y

Bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. The etiologic agents of these diseases are Flavobacterium (F.) psychrophilum, Yersinia (Y.)...
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Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR

Moon HJ, Kim HK, Park SJ, Lee CS, Song DS, Kang BK, Park BK

Porcine endogenous retroviruses (PERVs) are members of family Retroviridae, genus Gamma retrovirus, and transmitted by both horizontally and vertically like other endogenous retroviruses (ERVs). PERV was initially described in the...
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The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates

Kim TJ, Lee JI

Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates,...
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Detection of Streptococcus dysgalactiae subsp. equisimilis in equine nasopharyngeal swabs by PCR

Preziuso S, Laus F, Tejeda AR, Valente C, Cuteri V

Streptococcus (S.) dysgalactiae subsp. equisimilis is responsible for severe diseases in humans, including primary bacteraemia, pneumonia, endocarditis, and toxic shock syndrome. Infection in some animal species can also occur, although...
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