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Simultaneous Three Color Detection of Surface Antigen (My 7), Intracellular Antigen (c-myc), and DNA Content using Single Laser Flow Cytometry

Han KT, Ryu KS, Namkoong S, Kim SP, Rha JG, Song SK, Kim SJ, Lee HY, Parker J

  • KMID: 2260727
  • Korean J Obstet Gynecol.
  • 1997 Jan;40(1):181-190.
Flow cytometry, a useful tool for measuring DNA content and cell differentiation as expressed by cell surface markers, is utilized to measure multiple antigens, especially surface antigen, intracellular oncoprotein, and...
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The Experience of HLA-B27 Test Using Flowcytometry

Shin BM

  • KMID: 2325361
  • J Lab Med Qual Assur.
  • 2002 Dec;24(2):209-213.
BACKGROUND: HLA-B27 is associated with an increased incidence of specific spondyloarthropathies(SpA), most notably ankylosing spondylitis(AS). I evaluated the cases referred for HLA-B27 antigen using flowcytometry (FCM) to find the clinical...
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Normal Lymphocyte Subpopulation of the Spleen is Altered after Peripheral Nerve Injury in Mice

You JH, Song HK, Jeong DC, Hyoun BD

BACKGROUND: Chronic neuropathic pain is often associated with altered immune function and the modulated immune cell response play a role in neuropathic pain by experimental nerve injury. In order to...
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Expression Patterns of Immunologic Surface Markers in Acute Leukemia

Choi HS, Kim KY, Lee JW, Suh JS, Lee WK, Kim JS, Jean DS

  • KMID: 2083356
  • Korean J Hematol.
  • 1997 May;32(1):86-97.
BACKGROUND: Immunophenotyping is an important technique for the diagnosis and classification of acute leukemia, as well as French-American-British (FAB) classification on the basis of morphologic characteristics and cytochemistry. We...
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Expression Pattern of the Thioredoxin System in Human Endothelial Progenitor Cells and Endothelial Cells Under Hypoxic Injury

Park KJ, Kim YJ, Choi EJ, Park NK, Kim GH, Kim SM, Lee SY, Bae JW, Hwang KK, Kim DW, Cho MC

BACKGROUND AND OBJECTIVES: The thioredoxin (TRx) system is a ubiquitous thiol oxidoreductase pathway that regulates cellular reduction/oxidation status. Although endothelial cell (EC) hypoxic damage is one of the important pathophysiologic...
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Flow Cytometric Detection of Apoptosis and p53 Protein in OVCAR-3 and SKOV-3 Ovarian Cancer Cell Lines

Yoon JH, Choi WM, Jo YS, Rha JG, Han KT

  • KMID: 2272089
  • Korean J Obstet Gynecol.
  • 2003 Jul;46(7):1279-1287.
OBJECTIVE: Taxol (paclitaxel)-induced apoptosis was studied to understand their biological mechanism correlated with the expression of p53 in the SKOV-3 and OVCAR-3 ovarian cancer cell lines. MATERIALS AND METHODS: The SKOV-3...
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Detection of Apoptosis by the Stainings of Annexin V, Propidium Iodide and Cytokeratin in OVCAR-3 Ovarian Cancer Cell Line

Song MK, Kwon MK, Lee JW, Choi YH, Kim TW, Ryu KS, Rha JG, Han KT

  • KMID: 2272096
  • Korean J Obstet Gynecol.
  • 2003 Jul;46(7):1332-1340.
OBJECTIVE: This study was designed to estimate the chemosensitivity by a quantitative evaluation of the apoptotic cell fractions using flow cytometry. METHODS: The OVCAR-3 cells were exposed to 20 nM or...
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Characterization of Peripheral Blood CD34+ Cells Mobilized by Recombinant Human Granulocyte-Colony Stimulating Factor in Healthy Adult Donors

Byun JR, Chung IJ, Kwon SY, Seo JS, Choi KS, Park MR, Lee JJ, Kim HJ

  • KMID: 1849145
  • Korean J Hematol.
  • 1998 Oct;33(3):411-420.
BACKGROUND: CD34+ cells are capable of engraftment and hematopoietic reconstitution. However, expression patterns of other surface antigens such as CD13, CD33, CD38 and HLA-DR on CD34+ cells in mobilized peripheral...
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Multiparametric Flow Cytometry in Breast Cancer Cell Line (MCF-7) Stained with Fluorescein Isothiocyanate, Phycoerythrin, and Propidium Iodide

Han KT, Ryu KS, Han SH, In K, Song JM, Kim JH, Lee JK, Rha JG, Kim SP, Lee HY

  • KMID: 2175123
  • J Korean Cancer Assoc.
  • 1999 Dec;31(6):1129-1139.
PURPOSE: Multiparametric flow cytometry is a powerful tool for analyzing the phenotypic, cell kinetic and ploidy heterogeneity of tumor cell populations. But there are major problems such as inaccurate results...
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