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Characterization of an Extracytoplasmic Chaperone Spy in Protecting Salmonella against Reactive Oxygen/Nitrogen Species

Park YM, Lee HJ, Bang IS

Antimicrobial actions of reactive oxygen/nitrogen species (ROS/RNS) derived from products of NADPH oxidase and inducible nitric oxide (NO) synthase in host phagocytes inactivate various bacterial macromolecules. To cope with these...
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Expression, Characterization and Chain Shuffling of an Anti-HBsAg Phage Antibody

Choi IH, Kim IJ, Choi SJ, Kim HJ, Chung JH, Yi KS, Lee SD, Suh PG

  • KMID: 1551644
  • J Korean Soc Microbiol.
  • 1998 Jun;33(3):307-316.
In our previous report an anti-HBsAg human monoclonal antibody was generated using antibody phage display library technique. Using pComb3 filamentous phagemid vector, Fab molecule was expressed in fusion form to...
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Production of the Recombinant Single Chain Anti-B Cell Lymphoma Antibody and Evaluation of Immunoreactivity

Jung JH, Choi TH, Woo KS, Chung WS, Kim SG, Cheon GJ, Choi CW, Lim SM

  • KMID: 1512532
  • Nucl Med Mol Imaging.
  • 2006 Aug;40(4):211-217.
PURPOSE: Recombinant ScFv lym-1 was produced, using pET vector system for large scale production. METHODS: ScFv lym-1 gene inserted pET-22b (+) vector, was expressed in E.coli BL-21 strain. ScFv lym-1 antibody...
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Electron microscopic observation of Chlamydia pneumoniae

Lee S, Keum DG, Choi TY

  • KMID: 2039329
  • Korean J Clin Pathol.
  • 1997 Feb;17(1):146-154.
BACKGROUND: Chlamydia pneumoniae causes pneumonia and upper respiratory tract infection and has been recently reported to be associated with coronary atherosclerosis. The difference between C. pneumoniae and other Chlamydia spp....
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Production of Mouse Single Chain Fv Antibody to Surface Protein of Hepatitis B virus using Antibody Phage Display Library

Choi IH, Kim HS, Kim IJ, Chung JH, Park SK

  • KMID: 2001267
  • J Korean Soc Microbiol.
  • 1997 Aug;32(4):447-454.
In this study, we are to produce the single chain variable fragment (scFv) antibodies against surface protein of hepatitis B virus (HBV) using antibody phage display technique. Balb/c mice were...
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Proteomic Analysis of Thiol-active Proteins of Helicobacter pylori 26695

Park JW, Song JY, Hwang HR, Park HJ, Youn HS, Seo JH, Kang HL, Lee KH, Baik SC, Lee WK, Cho MJ, Rhee KH

Helicobacter pylori are a capnophilic bacterium, which colonize gastric mucosa and are resistant to acidic and oxidative damage. Thiol-active proteins subserve redox functions in tolerating oxidative stress and environmental toxicants,...
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