- Characterization of denaturation and renaturation of DNA for DNA hybridization
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Wang X, Lim HJ, Son A
- KMID: 2380100
- Environ Health Toxicol.
- 2014 ;29(1):e2014007.
- doi: 10.5620/eht.2014.29.e2014007
OBJECTIVES: The present study was designed to systematically characterize the denaturation and the renaturation of double stranded DNA (dsDNA), which is suitable for DNA hybridization. METHODS: A series of physical and... -
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- Ultrastructural In Situ Hybridization Method for Detection of Oncogenic Viruses
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Kee KH, Choi CH, Jeon HJ
- KMID: 2366500
- Korean J Pathol.
- 1996 Aug;30(8):687-698.
In situ hybridization (ISH) is a standard method for localizing DNA or RNA sequences in tissue or cell preperation. The technique was developed at the electron-microscopic level, and enables the... -
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- Melting of complexes of DNA-cis-DDP in acidic environment
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Dalyan YB, Haroutiunian TS, Haroutiunian SG, Vardevanyan PO
- KMID: 1119335
- Exp Mol Med.
- 2003 Dec;35(6):534-537.
The peculiarities of helix-coil transition of DNA at complex-formation with cis-diamminedichlorplatinum (II) (cis-DDP) have been investigated in acidic environment by UV absorbance technique. It is shown that observed features of... -
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- Detection of Vancomycin-resistant Enterococci using Multiplex Real-time PCR Assay and Melting Curve Analysis
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Cha CH, An HK, Kim JU
- KMID: 1096799
- Korean J Lab Med.
- 2010 Apr;30(2):138-146.
- doi: 10.3343/kjlm.2010.30.2.138
BACKGROUND: We developed and evaluated the utility of a multiplex real-time PCR assay that uses melting curve analysis and allows simultaneous identification of vancomycin-resistant genotypes and clinically relevant enterococci. METHODS: The... -
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- Usefulness of Multiplex Real-Time PCR and Melting Curve Analysis in Identification of Nontuberculous Mycobacteria
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Kang SH, Yoo KC, Park KU, Song J, Kim EC
- KMID: 1121658
- Korean J Lab Med.
- 2007 Feb;27(1):40-45.
- doi: 10.3343/kjlm.2007.27.1.40
BACKGROUND: Nontuberculous mycobacteria (NTM) should be correctly identified to the species level, because of different treatment plans among NTM species. This study was performed to assess the usefulness of... -
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