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In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes

Khatun M, Bhuiyan MM, Ahmed JU, Haque A, Rahman MB, Shamsuddin M

Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries...
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Post-thaw development of in vitro produced buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification

Manjunatha BM, Ravindra J, Gupta PS, Devaraj M, Honnappa T, Krishnaswamy A

The present study was conducted to examine post-thaw in vitro developmental competence of buffalo embryos cryopreserved by cytoskeletal stabilization and vitrification. In vitro produced embryos were incubated with a medium...
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Dynamic analysis of Ca2+ level during bovine oocytes maturation and early embryonic development

Liang SL, Zhao QJ, Li XC, Jin YP, Wang YP, Su XH, Guan WJ, Ma YH

Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca2+ and its dynamic changes in the...
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Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid

Jang G, Hong SG, Lee BC

Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated...
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