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A Study of in situ PCR in the Tissue Sections of Borderline Tuberculoid Leprosy without the Bacilli under the Fite Stain

Choi HY

In situ PCR was applied to the paraffin embedded tissue section of two borderline tuberculoid patients which did not show acid fast bacilli on the Fite stain. In situ PCR...
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Modified Method of Multilocus Sequence Typing (MLST) for Serotyping in Salmonella Species

Yun YS, Chae SJ, Na HY, Chung GT, Yoo CK, Lee DY

Multilocus sequence typing (MLST) of Salmonella is useful method for replacing serotyping using antisera but is limited by difficulties associated with in polymerase chain reaction (PCR). We optimized the PCR...
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Serum Indeces Protein Fibrils Expression in Candida albicans

Koh CM, Lee KH, Shin WS, Kim DH

  • KMID: 2146909
  • J Korean Soc Microbiol.
  • 1999 Jun;34(3):277-283.
The fibrillar coat of Candida albicans is of interest as its significance in antigenicity, antiphagocytosis, and adherence to host tissues. The partial biochemical properties and ultrastructure of fibrillar coat induced...
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A Study of in situ PCR on Borderline Leprosy Patients

Choi HY

This study was planned to help the diagnosis of borderline leprosy by application of the in situ PCR. In situ PCR was performed in the tissue from skin biopsy using...
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Interleukin-10 Gene Polymorphism in patients with Schizophrenia

Pae CU, Park JM, Kim KS

  • KMID: 2340787
  • J Korean Neuropsychiatr Assoc.
  • 2002 Nov;41(6):972-979.
OBJECT: This study was aimed at comparing the Interleukin-10 gene polymorphic variants of patients with schizophrenia with those of normal controls, to investigate its contribution to the development of schizophrenia. METHOD:...
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Aflatoxin Related p53 Codon 249 Point Mutation in Korean Hepatocellular Carcinoma

Wang HJ, Hong SW, Park YS, Kim WH, Kim MW

  • KMID: 1928537
  • Korean J Hepatobiliary Pancreat Surg.
  • 1998 Feb;2(1):79-83.
PURPOSE: P53 point mutation, especially G to T transversion at codon 249, has been found in many cases of the aflatoxin-related hepatocellular carcinoma(HCC). This study was aimed to exam the...
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Identification of the Proteinase K-resistant Antigen of Orientia tsutsugamushi by Monoclonal Antibodies

Kim MK, Kim MJ, Lee SM, Kang JS

Orientia tsutsugamushi, the causative agent of scrub typhus, is an obligate intracellular bacterium that replicates in the cytosol of host cells. Although several protein antigens have been characterized and cloned,...
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The Comparison of Plankton Detection by Two Analysis Methods in the Seawater of Gageo Island

Hong JW, Lee KL, Kim YS

The acid digestion method for extracting diatoms has been widely used to confirm death by drowning, but its reliability is still disputed because some diatoms can be destroyed during the...
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New DNA Extraction Method for Diagnosis of Tuberculosis by Polymerase Chain Reaction

Seo DY, Son HC, Kim SH

  • KMID: 2039325
  • Korean J Clin Pathol.
  • 1997 Feb;17(1):109-119.
BACKGROUND: To assess the clinical utility of new DNA extraction method, the authors attempted PCR using mycobacterial DNA extracted by Chelex 100 ion exchange resin method for 63 clinical samples...
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The Result of Mitochondrial DNA Deletion from PCR Analysis of Peripheral Blood for Parkinson's Disease

Cha SK, Yoo YM, Lee U

  • KMID: 1955535
  • J Korean Neurosurg Soc.
  • 1998 Aug;27(8):1023-1028.
Parkinson's disease(PD) is a neurodegenerative disorder characterised clinically by bradykinesia, rigidity, tremor, and pathologically by neuronal cell death in substantia nigra. The cause of dopaminergic neuronal cell death in PD...
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Improved Technique of Digoxigenin Labeled RNA in situ Hybridization

Lee SK, Kim YS, Song IS, Lee SS, Lee YJ, Kim WH, Chi JG

  • KMID: 2275639
  • Korean J Pathol.
  • 2001 Apr;35(2):98-110.
BACKGROUND: A practical RNA in situ hybridization method using digoxigenin labeled RNA probes is described in order to evaluate the technical difficulties and problems in RNA in situ hybridization. METHODS:...
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The Analysis of Reclaiming Ratio for 3 Diatom Species from Experimentally Drowned Animal Organs

Hong JW, Kim YS

Drowning is one of the most common causes accidental death worldwide, but its diagnosis remains a challenging task in forensic pathology. Several authors have suggested that diatom analysis be conducted...
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Detection of Herpes Viral DNA in Peripheral Blood of Kawasaki Disease Patient by Nested Polymerase Chain Reaction

Kim HS

  • KMID: 2207471
  • J Korean Pediatr Soc.
  • 2000 Sep;43(9):1180-1186.
PURPOSE: Epidemiologic evidence suggests an infectious cause for Kawasaki disease(KD), but the etiology of KD remains unknown. The aim of this study was to determine whether viral infections are related in the...
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Helicobacter pylori Urease Induces Mouse Death

Baik SC, Kang HL, Seo JH, Park ES, Rhee KH, Cho MJ

  • KMID: 1574158
  • J Bacteriol Virol.
  • 2005 Sep;35(3):175-181.
The component of Helicobacter pylori responsible for mouse death was identified and partially characterized. Mice that were injected with H. pylori cell lysate showed pathological changes such as decreased activity,...
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Analysis of Mitochondrial and Y-chromosomal DNA from 350-Year-Old Mummified Human Tissue

Shin KJ, Yang YS, Han GR, Kim CY

  • KMID: 1849282
  • Korean J Leg Med.
  • 2003 Oct;27(2):49-55.
This paper describes the successful DNA extraction and amplification, and analysis of mitochondrial and Y-chromosomal DNA from an approximately 350-year-old mummy exhumed from Gyunggi-do, South Korea in 2001. Sample tissue...
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Association Study between Schizophrenia and Monocyte Chemoattractant Protein-1(MCP-1) Gene Polymorphism

Chung KI, Pae CU, Jo JH, Kim JJ, Lee CU, Lee SJ, Lee C, Paik IH, Bang SK

  • KMID: 1636026
  • J Korean Neuropsychiatr Assoc.
  • 2003 Jul;42(4):454-460.
OBJECTIVES: This study was aimed at comparing the Monocyte Chemoattractant Protein-1 (MCP-1) gene polymorphic variants of schizophrenics with those of normal controls, and to investigate its contribution to the development...
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Gender Verification Test Based on PCR Determination of Y Chromosomal DNA: Experience in Genetic Sex Typing during the '99 Kangwon Asian Winter Games

Kim JU, Herr H, Chang HK, Ahn HK, Oh KJ, Kim HK

  • KMID: 2225941
  • Korean J Clin Pathol.
  • 2000 Apr;20(2):215-246.
BACKGROUND: The aim of gender verification test is to maintain impartiality among female competitors by excluding males in women's sports competitions. Some microscopic methods such as X-chromatin test and Y-chromatin...
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An Alternative Method for Extracting Plasmodium DNA from EDTA Whole Blood for Malaria Diagnosis

Seesui K, Imtawil K, Chanetmahun P, Laummaunwai P, Boonmars T

Molecular techniques have been introduced for malaria diagnosis because they offer greater sensitivity and specificity than microscopic examinations. Therefore, DNA isolation methods have been developed for easy preparation and cost...
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Amplification of distinct α-synuclein fibril conformers through protein misfolding cyclic amplification

Jung BC, Lim YJ, Bae EJ, Lee JS, Choi MS, Lee MK, Lee HJ, Kim YS, Lee SJ

Amyloid fibril formation has been implicated in the pathogenesis of neurodegenerative diseases. Fibrillation generates numerous conformers. Presumably, the conformers may possess specific biological properties, thus providing a biochemical framework for...
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Protective Effects of Immunization with Proteinase, Phospholipase and Cell Wall Proteins of Candida albicans in Mice Candidosis

Shin WS, Choi SJ, Yang YC, Park SJ, Park JY, Kim SK, Lee KH

  • KMID: 1474500
  • Korean J Med Mycol.
  • 2009 Mar;14(1):9-15.
BACKGROUND: The opportunistic fungus Candida albicans is a major pathogen especially to immunocompromised patients. OBJECTIVES: We examined the protective effect of the active and passive immunizations to evaluate the applicability for...
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