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Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Varshney B, Ponnanna N, Sarkar PA, Rehman P, Shah JH

Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies...
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Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

Choi IS, Yoo HS

  • KMID: 1095123
  • J Vet Sci.
  • 2002 Sep;3(3):207-212.
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2...
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Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine

El Hakim A, Shahein YE, Abdel-Shafy S, Abouelella AMK, Hamed RR

In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval...
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Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

Shin SJ, Bae JL, Cho YW, Yang MS, Kim DH, Jang YS, Yoo HS

  • KMID: 1089601
  • J Vet Sci.
  • 2003 Dec;4(3):225-228.
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by...
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Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice

Lim JJ, Kim DH, Lee JJ, Kim DG, Min W, Lee HJ, Rhee MH, Kim S

The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study,...
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Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Lee DY, Cho YW, Kang SG, Shin SJ, Yoo HS

  • KMID: 1093629
  • J Vet Sci.
  • 2004 Dec;5(4):337-343.
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and...
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Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)

Choi MH, Ryu JS, Lee MJ, Li S, Chung BS, Chai JY, Sithithaworn P, Tesana S, Hong ST

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major...
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