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Improved assessment of frozen/thawed mouse spermatozoa using fluorescence microscopy

Diercks AK, Burgers HF, Schwab A, Schenkel J

Genetically modified (GM) animals are unique mutants with an enormous scientific potential. Cryopreservation of pre-implantation embryos or spermatozoa is a common approach for protecting these lines from being lost or...
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Alginate encapsulation preserves the quality and fertilizing ability of Mediterranean Italian water buffalo (Bubalus bubalis) and Holstein Friesian (Bos taurus) spermatozoa after cryopreservation

Perteghella S, Gaviraghi , Cenadelli , Bornaghi V, Galli A, Crivelli B, Vigani B, Vigo D, Chlapanidas T, Faustini M, Torre ML

The use of artificial insemination (AI) in buffalo (Bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells...
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Transmission electron microscopy for characterization of acrosomal damage after Percoll gradient centrifugation of cryopreserved bovine spermatozoa

Oliveira L, Hossepian de Lima VFM, Levenhagen MA, dos Santos RM, Assumpcao , Jacomini J, de Andrade AFC, de Arruda , Beletti ME

The objective of this study was to characterize acrosomal ultrastructure following discontinuous Percoll gradient centrifugation of cryopreserved bovine sperm. Semen was collected from six bulls of different breeds and three...
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