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Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs

van Wyngaardt W, Mashau C, Wright I, Fehrsen J

There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody...
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Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Zhao H, Wang Y, Ma Z, Wang Y, Feng WH

Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly,...
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Dietary germanium biotite supplementation enhances the induction of antibody responses to foot-and-mouth disease virus vaccine in pigs

Lee JA, Jung BG, Jung M, Kim TH, Yoo HS, Lee BJ

We evaluated the potential ability of germanium biotite (GB) to stimulate the production of antibodies specific for foot-and-mouth disease virus (FMDV). To this aim, we measured the total FMDV-specific antibody...
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Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

Li H, Yang J, Bao D, Hou J, Zhi Y, Yang Y, Ji P, Zhou E, Qiao S, Zhang G

A simple and rapid immunochromatographic test strip incorporating a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies...
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Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43

Lee HK, Lee BH, Seok SH, Baek MW, Lee HY, Kim DJ, Na YR, Noh KJ, Park SH, Kumar DN, Kariwa H, Nakauchi M, Heo SJ, Park JH

Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative...
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